Yinsheng Li, Hao Tang, Xiuhong Wang, Weiguo Qiu, J. Qiu
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引用次数: 0
摘要
砷酸是动物养殖中常用的饲料添加剂。几乎没有研究关注它对蚯蚓体内酶活性的影响。本研究对暴露于砷酸环境下的蚯蚓细胞色素P450系统中三种重要酶的活性进行了研究:苯氧基-间苯二酚- o -脱烷基酶(BROD)、戊氧基间苯二酚- o -脱烷基酶(PROD)、甲氧基间苯二酚- o -脱烷基酶(MROD)。设计了5个不同浓度的氨基苯甲酸基团，模拟氨基苯甲酸随动物粪便排入土壤的过程。分别测定暴露后不同时间线虫体内BROD、PROD、MROD的活性。结果表明，这三种酶均被砷酸抑制。抑制程度为BROD>;PROD>;MROD。它还显示了一些治疗的时间或剂量依赖性。结果表明，这3种酶可作为土壤中砷酸污染的生物标志物。
Notice of RetractionResponse of Cytochrome P450 in Earthworms Exposed to Arsanilic Acid Contaminated Soils
Arsanilic acid is a normal used feed additive in animal breeding. Almost no studies have concerned its effect on the enzymatic activities in earthworms. In present study, we investigated the activities of three important enzymes in cytochrome P450 system: benzoxy- resorufin-O -dealkylase (BROD), Pentoxyresorufin -O- dealkylase (PROD), Methoxyresorufin-O -deethylase (MROD) in earthworm Eisenia andrei exposed to arsanilic acid. 5 different concentration groups of arsanilic acid were designed to simulate the process that arsanilic acid was discharged into soil with animal manure. BROD, PROD, MROD activities in worms were respectively measured in different time after exposure. The results showed that all the three enzymes in worms were inhibited by arsanilic acid. The inhibition extents were BROD>;PROD>; MROD. It also showed time or dose dependent is some treatments. As a conclusion, the three enzymes might be used as biomarker of arsanilic acid contamination in soils.
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