从N-heksana提取物中提取的N-heksana叶绿素,并测试己球菌菌和Escherichia大肠杆菌的抗菌活性

Yuszda K. Salimi
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引用次数: 5

摘要

辣木(Moringa oleifera Lamk)是一种在热带气候中自然生长的植物。在这个过程中,人们用植物的叶子作为抗菌剂。本研究的目的是从kelor叶的乙酸乙酯萃取物中分离出三萜化合物。以甲醇为溶剂,采用浸渍法提取样品。分馏采用正己烷和乙炔乙烷作为溶剂。采用柱层析法对该化合物进行分离纯化,然后采用薄层色谱法进行染色模式分析。采用UV、IR、1H-NMR、12C-NMR、HSQC、HMBC、1H-1H COSY等方法对纯化合物结构进行表征和解析,并与各种文献进行比较。根据结构解析的结果,得到的纯分离物为五环三萜基团化合物,分子式为C30H50O,名称为3-羟基,20 (29)-en, lupenol。3-羟氧基,20 (29)-en, lupenol是首次从kelor植物叶片中分离报道的三萜化合物。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Senyawa Triterpenoid dari Ekstrak N-heksana Daun Kelor (Moringa oleifera Lamk.) dan Uji Aktivitas Antibakteri Terhadap Staphylococcus aureus dan Escherichia coli
Kelor plant (Moringa oleifera Lamk) is a plant that grows naturally in a tropical climate. During this decoction of pirdot leaves plants are used by the people as antimicroba. The purpose of this study was to isolate triterpenoid compound from ethyl acetate extract of kelor leaves. The sample extraction was performed by maceration method using methanol as a solvent. Fractionation used n-hexana and etil asetat as a solvent. The separation and purification of the compound was carried out by column chromatography method followed by stain pattern analysis with thin layer chromatography (TLC). Characterization and elucidation of pure compound structures using UV, IR, 1H-NMR, 12C-NMR, HSQC, HMBC, and 1H-1H COSY, and compared with various literatures. Based on the result of structural elusidation, the pure isolate obtained is a pentacyclic triterpenoid group compound with the molecular formula C30H50O and the name of 3-hydroxy, 20 (29) -en, lupenol. The triterpenoid compound of 3-hidroxy, 20 (29) -en, lupenol is the first isolated and reported from leaves of kelor plant.
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