用蜜蜂酯酶提取物检测有机磷和氨基甲酸酯类农药的颜色测试试剂盒

Q3 Multidisciplinary
Udomsap Jaitham, Sumed Yadoung, Anurak Wongta, B. Chuttong, K. Danmek, Surat Hongsibsong
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引用次数: 0

摘要

有机磷酸酯(OPs)和氨基甲酸酯(CMs)是两类农药,广泛用于农业、公共卫生和家庭害虫防治。OPs和CMs都通过抑制乙酰胆碱酯酶的活性起作用,乙酰胆碱酯酶是一种分解神经递质乙酰胆碱的酶。用于检测OPs和CMs的分析方法可能很昂贵,因为它们通常需要专门的设备和经验丰富的分析人员。因此,有必要开发低成本,快速,简单的分析技术来检测OPs和CMs。本研究建立了一种利用蜜蜂头部酯酶和比色法检测OPs和CMs的方法。有机磷农药的检出限(lod)分别为敌敌畏、美威磷、噻虫磷和毒死蜱,检出限分别为0.005、0.001、0.02和0.02 mg/kg;有机磷农药的检出限(lod)分别为威威、硫虫威、恶氨酰和敌敌畏,检出限分别为0.0008、0.03、0.01和0.09 mg/kg。结果表明,该方法与GC-FPD法在检测果蔬中有机磷农药残留量方面无显著差异。该方法可用于果蔬中OPs和CMs残留的检测,降低了检测成本,验证简便。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Color Test Kit for Detecting Organophosphate and Carbamate Pesticides by Using Esterase Extract from Honey Bees
Organophosphates (OPs) and carbamates (CMs) are two classes of pesticides that are widely used in agriculture, public health, and home pest control. Both OPs and CMs work by inhibiting the activity of acetylcholinesterase, an enzyme that breaks down the neurotransmitter acetylcholine. Analytical methods used for the detection of OPs and CMs can be costly, as they often require specialized equipment and experienced analysts. Therefore, it is necessary to develop low-cost, quick, and simple analytical techniques for OPs and CMs detection. This study developed a method for detecting OPs and CMs using esterase enzymes from honey bee heads and a colorimetric technique. The limits of detection (LODs) for OPs include dicrovos, mevinphos, azinophos-methyl, and chlorpyrifos with values of 0.005, 0.001, 0.02, and 0.02 mg/kg, respectively, and the LODs for CMs are carbaryl, ethiofencarb, oxamyl, and fenobucarb with values of 0.0008, 0.03, 0.01, and 0.09 mg/kg, respectively. Comparisons between the developed method and the GC-FPD showed no difference in detecting OPs and CMs residues in fruits and vegetables. This developed method can detect OPs and CMs residues in fruits and vegetables, which will help reduce costs and facilitate easy verification.
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来源期刊
Philippine Journal of Science
Philippine Journal of Science Multidisciplinary-Multidisciplinary
CiteScore
1.20
自引率
0.00%
发文量
55
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