含甲酰胺嘧啶的寡核苷酸的合成与表征。dA, fappy . dg)。

Nucleic acids research. Supplement Pub Date : 2001-11-01 DOI:10.1093/NASS/1.1.129

K. Haraguchi, M. Delaney, C. Wiederholt, A. Sambandam, Z. Hantosi, M. Greenberg

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引用次数: 6

摘要

含fpy寡核苷酸的制备。dA (N4-(2-脱氧- α， β -d -红-戊呋喃基)-4,6-二氨基- 5-甲脒嘧啶)和Fapy。dG (N6-(2- deoxy - α， β - d - red -戊-呋喃基)-2,6-二氨基-4-羟基-5-甲脒-嘧啶)是通过将病变引入二核苷酸磷酰胺而获得的。通过固相合成和/或化学合成和酶连接相结合的方法制备了由多达36个核苷酸组成的寡核苷酸。还制备了含有不可水解类似物的寡核苷酸。含有这些修饰的核苷酸的寡核苷酸通过各种化学和生化方法进行了表征。

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Synthesis and characterization of oligonucleotides containing formamidopyrimidine lesions (Fapy.dA, Fapy.dG) at defined sites.

The preparation of oligonucleotides containing Fapy.dA (N4-(2-Deoxy-alpha,beta-D-erythro-pentofuranosyl)-4,6-diamino- 5-formamidopyrimidine) and Fapy.dG (N6-(2-Deoxy-alpha,beta-D-erythro-pento-furanosyl)-2,6- diamino-4-hydroxy-5-formamido-pyrimidine) at defined sites was achieved by introducing the lesions as dinucleotide phosphoramidites. Oligonucleotides as composed of as many as 36-nucleotides were prepared by solid-phase synthesis and/or a combination of chemical synthesis and enzymatic ligation. Oligonucleotides containing non-hydrolyzable analogues were also prepared. Oligonucleotides containing these modified nucleotides were characterized by a variety of chemical and biochemical methods.

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Nucleic acids research. Supplement

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