桔皮水醇提取物对卵巢扭转大鼠组织损伤和氧化应激中PCNA和FSH-R基因表达的保护作用

IF 0.8 Q4 REPRODUCTIVE BIOLOGY
Seyedeh-Roza Tafrishi Nejad, A. Khaki, Shamci Abbasalizadeh, M. Shokoohi, Nava Ainehchi
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引用次数: 2

摘要

目的:探讨柑桔皮水酒精提取物对卵巢扭转致大鼠组织学损伤和酸应激时增殖细胞核抗原(PCNA)和促卵泡激素受体(FSH-R)基因表达的保护作用。材料与方法:选用32只成年雌性大鼠,随机分为4组。1组(Sham)切开腹壁,不施加扭转;2组卵巢扭转2小时,扭转2周。第三组在日粮中添加桔皮水酒精提取物2周,随后进行卵巢扭转2小时和卵巢扭转2小时。第4组大鼠连续2周服用橘皮提取物,切除卵巢后进行组织损伤评估,并采血检测血清抗氧化酶水平、卵巢组织中PCNA和FSH-R基因的表达。结果:大鼠卵巢组织组织学变化表明,扭转和扭转对卵巢组织有破坏作用,扭转和扭转导致PCNA和FSH-R表达降低(P < 0.05)。根据生物化学和激素结果,卵巢扭转导致大鼠氧化应激标志物和激素谱失衡。最后,由于橙皮的高抗氧化性能,使用水醇提取物可以改善这些效果。结论:一般来说,在饮食中连续两周给予适当剂量的橘皮水酒精提取物对卵巢组织有扭转/扭曲的保护作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Protective Effect of Hydroalcoholic Extract of Orange Peel on PCNA and FSH-R Gene Expression in Histological Damage and Oxidative Stress Due to Ovarian Torsion in Adult Rats
Objectives: The aim of this study was to evaluate the protective effect of the hydroalcoholic extract of orange peel on proliferating cell nuclear antigen (PCNA) and follicle-stimulating hormone receptor (FSH-R) gene expression in histological injuries and acid stress caused by ovarian torsion in adult rats. Materials and Methods: In this experimental study, 32 adult female rats were randomly divided into 4 groups. In group 1 (Sham), the abdominal wall was cut without applying torsion and in group 2, ovarian torsion was performed for 2 hours, followed by detorsion for 2 weeks. The hydro-alcoholic extract of orange peel was added to their diet for two weeks in group 3, followed by ovarian torsion for 2 hours and detorsion for 2 hours. Group 4 received the orange peel extract for two weeks and after then ovarian resection for the evaluation of histological damage and blood sampling to examine the serum level of antioxidant enzymes, as well as the expression of PCNA and FSH-R genes in the ovarian tissue. Results: Histological changes in the ovary tissue of rats showed that torsion and detorsion have destructive effects on the ovarian tissue, and torsion/detorsion led to a reduction in the expression of PCNA and FSH-R (P < 0.05). Based on biochemical and hormonal results, the ovarian torsion resulted in an imbalance in the oxidative stress markers and hormone profile of rats. Finally, the administration of the hydroalcoholic extract of orange peel due to its high antioxidant properties improves these effects. Conclusions: In general, administering an appropriate dose of the hydroalcoholic extract of orange peel for two consecutive weeks in the diet had a protective effect on the ovarian tissue at the risk of torsion/detorsion.
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来源期刊
CiteScore
1.60
自引率
14.30%
发文量
8
期刊介绍: All kind of knowledge contributing to the development of science by its content, value, level and originality will be covered by IJWHR. Problems of public health and their solutions are at the head of the windows opening us to the world. The "International Journal of Women''s Health and Reproduction Sciences” is a modern forum for scientific communication, covering all aspects women health and reproduction sciences, in basic and clinical sciences, mainly including: -Medical Education in Women Health and Reproduction Sciences -Cardiology in Women Health-Related Reproductive Problems -Sports Medicine in Women Health and Reproduction Sciences -Psychiatry in Women Health-Related Reproductive Problems -Antioxidant Therapy in Reproduction Medicine Sciences -Nutrition in Women Health and Reproduction Sciences -Defense Androgen and Estrogen -Fertility and Infertility -Urogynecology -Endometriosis -Endocrinology -Breast Cancer -Menopause -Puberty -Eroticism -Pregnancy -Preterm Birth -Vaginal Diseases -Sex-Based Biology -Surgical Procedures -Nursing in Pregnancy -Obstetrics/Gynecology -Polycystic Ovary Syndrome -Hyperandrogenism in Females -Menstrual Syndrome and Complications -Oncology of Female Reproductive Organs -Traditional Medicine in Women Reproductive Health -Ultrasound in Women Health Reproduction sciences -Stem Cell Research In Women Reproduction Sciences -Complementary Medicine in Women Reproductive Health -Female Sexual Dysfunction: Pathophysiology & Treatment
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