山参的抗氧化性能及其对正常和高血糖大鼠的降血糖作用

Irisappan Sarath Chandiran , Korlakunta Narasimha Jayaveera , Karimulla Shaik
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引用次数: 3

摘要

目的研究山黧豆(Grewia serrulata DC, aags &正常和高血糖雄性Wistar白化大鼠的脑电图。方法采用标准方法对其体外和体内模型的抗氧化性能进行评价。采用正常血糖和口服糖耐量试验(NG-OGTT)法,并分别以200和400 mg/kg体wt p.o剂量急性给予链脲佐菌素(STZ)诱导的高血糖大鼠,观察其降血糖活性。结果木参醇提物对DPPH(1,1-二苯基-2-苦味酰肼)(9.16±1.05 μg/ml)、超氧化物(35.59±1.6805 μg/ml)和一氧化氮(151.80±1.79 μg/ml)的IC50值较低,具有较强的自由基清除活性。在体内研究中,两种剂量的乙醇提取物均显示出显著的剂量依赖性增加(p <0.05至p <0.001),超氧化物歧化酶(SOD)和过氧化氢酶(CAT)水平显著降低(p <0.05至p <0.001)硫代巴比妥酸反应性物质(TBARS)的水平,与CCl4处理的肝脏和肾脏对照组相比。在NG-OGTT方法中,脑电图显示正常大鼠、葡萄糖负荷高血糖大鼠和stz诱导的高血糖大鼠的葡萄糖水平降低。结论eegs具有较强的抗氧化活性和较好的降糖活性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Antioxidant property of Grewia serrulata DC and its hypoglycemic effect on normal and hyperglycemic rats

Aim

The present study was aimed to evaluate the antioxidant property and hypoglycemic activity of aqueous and ethanolic extracts of aerial parts of Grewia serrulata DC (AEGS & EEGS) in normal and hyperglycemic male Wistar Albino rats.

Methods

The antioxidant property was evaluated both in vitro and in vivo model using standard procedures. The hypoglycemic activity was evaluated by normal glucose and oral glucose tolerance test (NG-OGTT) method and by acute administration of AEGS and EEGS extracts at doses 200 and 400 mg/kg body wt p.o in Streptozotocin (STZ) induced hyperglycemic rats.

Results

The ethanolic extract of Grewia serrulata DC exhibited strong free radical scavenging activity as evidenced by their low IC50 values in DPPH (1,1-diphenyl-2-picryl-hydrazyl) (9.16 ± 1.05 μg/ml), Superoxide (35.59 ± 1.6805 μg/ml) and nitric oxide (151.80 ± 1.79 μg/ml) methods. In in vivo study, treatment with ethanolic extract at both doses shows a significant dose dependent increase (p < 0.05 to p < 0.001) in the levels of superoxide dismutase (SOD) and Catalase (CAT) and a significant decrease (p < 0.05 to p < 0.001) in the levels of Thiobarbituric acid reactive substances (TBARS), when compared to CCl4 treated control in both liver and kidney. In NG-OGTT method EEGS shows reduction in glucose levels in normal rats and glucose loaded hyperglycemic rats and in STZ-induced hyperglycemic rats.

Conclusion

EEGS was found to possess strong antioxidant activity and good hypoglycemic activity compared to that of AEGS.

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