在FRET实验的关键步骤遵循

IF 0.8 4区化学 Q4 SPECTROSCOPY

Spectroscopy Pub Date : 2022-08-01 DOI:10.56530/spectroscopy.dt1779u1

W. R. Algar

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引用次数: 0

摘要

Förster共振能量转移(FRET)是荧光方法工具箱的一个多功能部分。这种在供体荧光团和受体发色团之间的穿越空间、无光子的能量转移过程可能最著名的是它作为一种“分子标尺”的用途，可以解决纳米尺度的距离。FRET也是一个流行的和有利的基础生物分子分析和传感器。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Key Steps to Follow in a FRET Experiment

Förster resonance energy transfer (FRET) is a versatile part of the toolbox of fluorescence methods. This through-space, photon-less energy transfer process between a donor fluorophore and an acceptor chromophore is perhaps most famous for its utility as a “molecular ruler” that can resolve nanometer-scale distances. FRET is also a popular and advantageous basis for biomolecular assays and sensors.

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来源期刊

Spectroscopy 物理-光谱学

CiteScore

1.10

自引率

0.00%

发文量

审稿时长

3 months

期刊介绍： Spectroscopy welcomes manuscripts that describe techniques and applications of all forms of spectroscopy and that are of immediate interest to users in industry, academia, and government.