Minato Nakamura, Hisayuki Tomochi, Kiyohiko Andoh, A. Nishimori, Yuto Suda, Y. Matsuura, Y. Iwamaru
{"title":"2017 - 2021年市售胎牛血清中牛病毒性腹泻病毒污染的检测","authors":"Minato Nakamura, Hisayuki Tomochi, Kiyohiko Andoh, A. Nishimori, Yuto Suda, Y. Matsuura, Y. Iwamaru","doi":"10.12935/jvma.75.e139","DOIUrl":null,"url":null,"abstract":"Bovine viral diarrhea virus (BVDV) is one of the high-risk agents for contamination in fetal bovine serum (FBS). In this study, we examined 41 FBS samples collected between 2017 and 2021 for BVDV contamination. Thirty-four of 41 samples (82.9%) were positive to pestivirus-specific RT-PCR, and 17 (41.5%) and 1 (2.4%) of 41 samples were seropositive using the neutralization test for BVDV1 and 2, respectively. Ten of the 41 samples that were RT-PCR-positive with a high ELISA titer were subjected to virus isolation; however, no infectious virus was detected. Our results showed that only 5 of the 41 FBS samples (12.2%) were both PCR-negative and BVDV-specific antibody-free. It is important to note that FBS potentially poses some risks associated with BVDV.","PeriodicalId":17329,"journal":{"name":"Journal of the Japan Veterinary Medical Association","volume":"61 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Inspection of Commercially Available Fetal Bovine Sera Collected between 2017 and 2021 for the Contamination of Bovine Viral Diarrhea Virus\",\"authors\":\"Minato Nakamura, Hisayuki Tomochi, Kiyohiko Andoh, A. Nishimori, Yuto Suda, Y. Matsuura, Y. Iwamaru\",\"doi\":\"10.12935/jvma.75.e139\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Bovine viral diarrhea virus (BVDV) is one of the high-risk agents for contamination in fetal bovine serum (FBS). In this study, we examined 41 FBS samples collected between 2017 and 2021 for BVDV contamination. Thirty-four of 41 samples (82.9%) were positive to pestivirus-specific RT-PCR, and 17 (41.5%) and 1 (2.4%) of 41 samples were seropositive using the neutralization test for BVDV1 and 2, respectively. Ten of the 41 samples that were RT-PCR-positive with a high ELISA titer were subjected to virus isolation; however, no infectious virus was detected. Our results showed that only 5 of the 41 FBS samples (12.2%) were both PCR-negative and BVDV-specific antibody-free. It is important to note that FBS potentially poses some risks associated with BVDV.\",\"PeriodicalId\":17329,\"journal\":{\"name\":\"Journal of the Japan Veterinary Medical Association\",\"volume\":\"61 1\",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2022-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of the Japan Veterinary Medical Association\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.12935/jvma.75.e139\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of the Japan Veterinary Medical Association","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.12935/jvma.75.e139","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Inspection of Commercially Available Fetal Bovine Sera Collected between 2017 and 2021 for the Contamination of Bovine Viral Diarrhea Virus
Bovine viral diarrhea virus (BVDV) is one of the high-risk agents for contamination in fetal bovine serum (FBS). In this study, we examined 41 FBS samples collected between 2017 and 2021 for BVDV contamination. Thirty-four of 41 samples (82.9%) were positive to pestivirus-specific RT-PCR, and 17 (41.5%) and 1 (2.4%) of 41 samples were seropositive using the neutralization test for BVDV1 and 2, respectively. Ten of the 41 samples that were RT-PCR-positive with a high ELISA titer were subjected to virus isolation; however, no infectious virus was detected. Our results showed that only 5 of the 41 FBS samples (12.2%) were both PCR-negative and BVDV-specific antibody-free. It is important to note that FBS potentially poses some risks associated with BVDV.
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