天牛乳酸菌的分离及其对大肠杆菌的抑菌活性

E. Mursyida, Fifi Candita, M. Faisal, Deinike Wanita Marwan
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摘要

腹泻是一种可能导致死亡的潜在异常事件。在发展中国家引起腹泻的最常见微生物是轮状病毒和大肠杆菌。治疗腹泻的一种方法是使用抗生素。然而,已知抗生素的使用会破坏正常胃肠道菌群的平衡,从而改变微生物群的组成。益生菌可以在抗生素治疗期间或之后给予平衡正常菌群。乳酸菌是一种能抑制病原菌生长的益生菌,常见于发酵食品中。本研究旨在从印尼廖内省坎帕榴莲中分离出乳酸菌,并检测其对大肠杆菌的抑菌活性。本研究首先采用多级稀释法从天牛中分离乳酸菌,然后通过菌落和细胞形态表征乳酸菌,最后采用琼脂孔扩散法检测其对大肠杆菌的抑菌活性。抗菌试验结果采用单因素方差分析和Bonferroni随访检验进行分析。LAB鉴定结果表明,4株LAB菌株菌落形态不同,包括球形革兰氏阳性菌和过氧化氢酶阴性菌。结果表明,菌株LAB1对大肠杆菌的抑制能力最强,平均抑制带直径为15.21 mm;菌株lab4的抑制带最小,平均抑制带直径为10.80 mm。单因素方差分析结果显示,4株乳酸菌对大肠杆菌的抑制作用差异显著,P值<0.05。在Bonferroni试验中,lab1和lab4菌株之间存在显著差异。由此可见,从天牛中分离的乳酸菌具有开发益生菌的潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
ISOLATION OF LACTIC ACID BACTERIA FROM TEMPOYAK AND ITS ANTIMICROBIAL ACTIVITY ON THE Escherichia coli
Diarrhea is a potential extraordinary event that can cause death. The most common microbes that cause diarrhea in developing countries are Rotavirus and Escherichia coli. One of the treatment of diarrhea is by giving antibiotics. However, the use of antibiotics is known to disrupt the balance of normal gastrointestinal flora, thereby changing the composition of the microbiota. Probiotics can be given to balance the normal flora during or after treatment with antibiotics. Lactic acid bacteria (LAB) is one of the probiotics that can inhibit the growth of pathogenic microbes and is commonly found in fermented foods. This study aimed to isolate LAB from tempoyak made from kampar durian, Riau Province, Indonesia and test its antimicrobial activity against Escherichia coli. The study was initiated by isolating LAB from tempoyak using the multilevel dilution method, followed by characterizing LAB by colony and cell morphology, and testing its antimicrobial activity against Escherichia coli using the agar well diffusion method. The antimicrobial activity test results were analyzed using one-way ANOVA and Bonferroni follow-up test. The results of LAB characterization showed that four LAB isolates had different colony morphology, including spherical Gram-positive bacteria and catalase-negative bacteria. The results of the antimicrobial activity test showed that LAB1 isolates had the highest ability to inhibit Escherichia coli with an average inhibition zone diameter of 15.21 mm and the lowest inhibition zone was found in LAB 4 isolates, which was 10.80 mm. The results of the one way ANOVA test showed a significant difference between the four LAB isolates in inhibiting Escherichia coli with P value <0.05. In the Bonferroni test, there were significant differences between LAB 1 and LAB 4 isolates. It can be concluded that LAB isolated from tempoyak has the potential to be a source of probiotics.
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