High-throughput applications of phage display in proteomic analyses

One of the key issues of molecular biology at the beginning of the 21^st century is the disclosure of complex interactions involved in cellular function. The availability of sequenced genomes sets the stage for functional analysis of the proteins. However, this task demands new technologies to warrant the characterisation of interactions at a higher pace. Here, we discuss phage display as one of the emerging technologies to address functional relationships between proteins and give examples of its use by focusing on antibody generation for protein expression analysis, discovery of novel disease markers and therapeutic targets, and mapping of protein–protein interactions.