PERAN蛋白血凝素毛杆菌肺炎链球菌54 kDa SEBAGAI黏附素

Journal of Health Science Pub Date : 2020-08-21 DOI:10.33086/jhs.v13i2.1442

D. Mufida, Adellia Fira Fa'idha, Zahrah Febianti

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引用次数: 0

摘要

收稿日期:2020年01月20日修稿日期:2020年05月6日在线发布日期:2020年08月介绍:毛菌具有一种粘附素，其作用是通过粘附过程感染宿主细胞。研究表明，54 kDa的肺炎链球菌毛蛋白是一种血凝素。本研究旨在探讨54 kDa的肺炎链球菌毛蛋白是否也具有粘附素的作用。方法:用毛刀分离肺炎链球菌。用SDS-PAGE分析蛋白分子量。分离得到分子量为54 kDa的蛋白，得到蛋白溶液。对不同浓度的蛋白溶液进行粘附试验，测定其粘附指数。结果:Pearson相关检验得到p值为0.036，相关系数R = -0.840，说明两个变量存在显著的负相关关系。回归分析显示R2 = 0.997，表示肺炎链球菌蛋白54 kDa浓度对粘附指数的影响为99.7%。结论:肺炎链球菌54 kDa血凝素蛋白是一种黏附蛋白。王晓明，王晓明，王晓明，等。毛霉黏附蛋白与毛霉黏附蛋白的关系。Penelitian sebelumnya menunjukkan bahwa蛋白;pili肺炎链球菌;54kda merupakan蛋白;肺炎链球菌毛杆菌血凝素(54 kDa)。方法:异隔离肺炎链球菌，孟古那肯菌毛切割器。Hasil podongan pili dilakukan SDS-PAGE untuk蛋白鉴定。蛋白分离分子54 kDa，二异isolasi sehingga menghasilkan larutan蛋白。粘连蛋白粘连剂，粘连剂，粘连剂，粘连剂，粘连剂。Hasil: Uji korelasi Pearson diperoleh nilai p值为0.036 (p < 0.05)， koefisien korelasi R= - 0.0840。Hasil ini menunjukkan bahwa kedua variable memiliki hubungan yang signfikan, sangat kuat dengan arah hubungan阴性。回归分析:R2 0.997, artinya 99,7 % konsentrasi蛋白pili 54 kDa肺炎链球菌黏附指数。kespulan:血凝素蛋白菌毛54 kDa肺炎链球菌merupakan蛋白粘连素

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PERAN PROTEIN HEMAGLUTININ PILI Streptococcus pneumoniae 54 kDa SEBAGAI ADHESIN

Received: January, 20, 2020 Revised: May, 6, 2020 Available online: August, 2020 Introduction: Pili has an adhesin that plays a role in the adhesion process in order to infect the host cell. Research shown that the 54 kDa S. pneumoniae pili protein is a hemagglutinin. This study was to examine whether the 54 kDa of S. pneumoniae pili protein also act as an adhesin. Method: S. pneumoniae was isolated using a pili cutter. The protein molecular weight was analyzed using SDS-PAGE. Protein with a molecular weight of 54 kDa was isolated to produce protein solution. Adhesion test was carried out on a protein solution with multilevel concentrations to determine the adhesion index. Result: Pearson correlation test obtained p-value of 0.036 and the correlation coefficient R = -0.840, these results indicate that the two variables have a significant negative relationship. Regression analysis showed R2 0,997, mean that 99.7% of the concentration of protein S. pneumoniae 54 kDa influenced the adhesion index. Conclusion: Therefore, it can be concluded that the hemagglutinin protein S. pneumoniae 54 kDa is an adhesin protein. A B S T R A K Latar Belakang: Pili memiliki protein adhesin yang berperan dalam proses adhesi untuk menginfeksi sel hospes. Penelitian sebelumnya menunjukkan bahwa protein pili S. pneumoniae 54 kDa merupakan protein hemaglutinin. Tujuan penelitian ini adalah menguji peran protein hemaglutinin pili S. pneumoniae 54 kDa sebagai adhesin. Metode: Pili S. pneumoniae diisolasi menggunakan alat pili cutter. Hasil potongan pili dilakukan SDS-PAGE untuk mengidentifikasi berat molekul proteinnya. Protein berat molekul 54 kDa diisolasi sehingga menghasilkan larutan protein. Larutan protein diuji adhesi dengan konsentrasi bertingkat untuk mengetahui indeks adhesi. Hasil: Uji korelasi Pearson diperoleh nilai p-value 0,036 (p < 0,05) dan koefisien korelasi R= -0,840. Hasil ini menunjukkan bahwa kedua variabel memiliki hubungan yang signifikan, sangat kuat dengan arah hubungan negatif. Analisis regresi didapatkan R2 0,997, artinya 99,7 % konsentrasi protein pili 54 kDa S. pneumoniae mempengaruhi indeks adhesi. Kesimpulan: protein hemaglutinin pili 54 kDa S. pneumoniae merupakan protein adhesin

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Journal of Health Science 医学-毒理学

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