利用葱属植物试验系统研究巴西理查菌提取物和馏分的抗增殖和基因毒性

IF 0.4 4区生物学 Q4 Agricultural and Biological Sciences

Iheringia Serie Botanica Pub Date : 2017-12-31 DOI:10.21826/2446-8231201772313

R. Dornelles, G. Leal, A. C. S. Decian, D. Santos, Gabriel A. Radiske, M. Manfron, S. Tedesco

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引用次数: 1

摘要

药用物种巴西理查迪亚(Richardia brasiliensis Gomes)具有具有相当药理活性的次生代谢物。本研究旨在通过对有丝分裂周期中对染色体的损伤和对细胞增殖的影响，确定巴西香地上部分提取物和组分的抗增殖能力和遗传毒性。在浓度为10µg/mL和500µg/mL时，评价提取物和馏分的抗增殖和遗传毒性作用，计算有丝分裂指数(MI)和染色体改变百分比(CA)，并进行χ 2检验。观察其抗增殖能力，以秋季粗提物效果最好，且正己烷和丁醇部位的心肌梗死指数低于阴性对照。所有处理都有CA基因毒性，但低于阳性对照的值。

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Antiproliferative and genotoxic potential from extracts and fractions of Richardia brasiliensis Gomes ( Rubiaceae ) by the Allium cepa L. test system

The medicinal species Richardia brasiliensis Gomes has secondary metabolites with considerable pharmacological activities. This study aims to determine the antiproliferative and genotoxic capacity of extracts and fractions obtained from the aerial parts of R. brasiliensis , in the Allium cepa test, on which one can detect the damages on chromosomes and the infl uence on cell proliferation during the mitotic cycle. The antiproliferative and genotoxic eff ect from extracts and fractions were evaluated at concentrations of 10 and 500 µg/mL and the mitotic index (MI) and the percentage of chromosomal alterations (CA) were calculated and used on the χ² test. It was observed antiproliferative capacity, the best results were from the autumn crude extract, and the hexane and butanol fractions with lower MI than the negative control. All treatments were genotoxic with CA, but with lower values than the ones found in the positive control.

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来源期刊

Iheringia Serie Botanica PLANT SCIENCES-

CiteScore

0.70

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： The Iheringia, Série Botânica is a publication edited by the Museu de Ciências Naturais, Fundação Zoobotânica do Rio Grande do Sul, since 1958. The name Iheringia is actually a tribute to Hermann von Ihering (a German researcher who became a Brazilian citizen) and his important studies in the Natural Sciences.The journal has the mission of publishing articles, reviews and original scientific notes that cover diverse areas in Botany, and may be written in Portuguese, Spanish and English. The journal is widely available in both international and national scientific community. The issues are published and distributed twice a year to 118 national institutions and 152 international institutions. The distribution is either free of charge or by exchange. Impac Factor JCR 2014/2015 = 0.263 Qualis CAPES 2015: B 4 (Biodiversity) B1 (Agricultural Science I) B1 (Environment)