L. T. Quyen, Nguyễn Thị Minh Thu, Le Thi Lan Phuong, N. Trang
{"title":"ss精子检测试剂盒与Halosperm检测试剂盒在精子DNA片段分析中的比较","authors":"L. T. Quyen, Nguyễn Thị Minh Thu, Le Thi Lan Phuong, N. Trang","doi":"10.31276/vjste.62(2).71-76","DOIUrl":null,"url":null,"abstract":": The objectives of this work were to accurately evaluate testing kits that analyse sperm DNA fragmentation in infertile men and to provide a comparison of an improved testing kit (SSSperm testing kit) to the existing Halosperm testing kit in an analysis of sperm DNA fragmentation. A cross-sectional study was conducted on 300 semen samples from infertile men with a sperm concentration ≥1 million/ml using the Bland-Altman, T-test, and Pearson test for statistical study. The SSSperm testing kit had a coefficient of variation of CV%=2.26%<5% and t tn =0.97<t c =2. The two methods have similar DNA fragmentation index (DFI) results (r=0.995; p<0.001). The difference between the results of the two kits was not statistically significant (p=0.236>0.05). In conclusion, the SSSperm testing kit for the analysis of sperm DNA fragmentation is qualified as determined from quantitative tests, and the SSSperm testing kit is equivalent to the Halosperm testing kit.","PeriodicalId":23548,"journal":{"name":"Vietnam Journal of Science, Technology and Engineering","volume":"63 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2020-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Comparison of the SSSperm testing kitwith the Halosperm testing kit in an analysis of sperm DNA fragmentation\",\"authors\":\"L. T. Quyen, Nguyễn Thị Minh Thu, Le Thi Lan Phuong, N. Trang\",\"doi\":\"10.31276/vjste.62(2).71-76\",\"DOIUrl\":null,\"url\":null,\"abstract\":\": The objectives of this work were to accurately evaluate testing kits that analyse sperm DNA fragmentation in infertile men and to provide a comparison of an improved testing kit (SSSperm testing kit) to the existing Halosperm testing kit in an analysis of sperm DNA fragmentation. A cross-sectional study was conducted on 300 semen samples from infertile men with a sperm concentration ≥1 million/ml using the Bland-Altman, T-test, and Pearson test for statistical study. The SSSperm testing kit had a coefficient of variation of CV%=2.26%<5% and t tn =0.97<t c =2. The two methods have similar DNA fragmentation index (DFI) results (r=0.995; p<0.001). The difference between the results of the two kits was not statistically significant (p=0.236>0.05). In conclusion, the SSSperm testing kit for the analysis of sperm DNA fragmentation is qualified as determined from quantitative tests, and the SSSperm testing kit is equivalent to the Halosperm testing kit.\",\"PeriodicalId\":23548,\"journal\":{\"name\":\"Vietnam Journal of Science, Technology and Engineering\",\"volume\":\"63 1\",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2020-06-30\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Vietnam Journal of Science, Technology and Engineering\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.31276/vjste.62(2).71-76\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Vietnam Journal of Science, Technology and Engineering","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.31276/vjste.62(2).71-76","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Comparison of the SSSperm testing kitwith the Halosperm testing kit in an analysis of sperm DNA fragmentation
: The objectives of this work were to accurately evaluate testing kits that analyse sperm DNA fragmentation in infertile men and to provide a comparison of an improved testing kit (SSSperm testing kit) to the existing Halosperm testing kit in an analysis of sperm DNA fragmentation. A cross-sectional study was conducted on 300 semen samples from infertile men with a sperm concentration ≥1 million/ml using the Bland-Altman, T-test, and Pearson test for statistical study. The SSSperm testing kit had a coefficient of variation of CV%=2.26%<5% and t tn =0.970.05). In conclusion, the SSSperm testing kit for the analysis of sperm DNA fragmentation is qualified as determined from quantitative tests, and the SSSperm testing kit is equivalent to the Halosperm testing kit.
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