利用CD44、NANOG和OCT4基因对Ciptomangun Kusumo国立医院1例患者结肠癌细胞进行表征

M. Abdullah, B. Bela, A. Syam, M. Simadibrata, Sofy Meilany, Firda Annisa, Dian Amirulloh, D. Makmun, A. A. Rani
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引用次数: 0

摘要

CD44是一种细胞粘附分子,被认为是一种癌症标志物,在肿瘤的进展和转移中起着重要作用。CD44在肿瘤发生中的作用是由于它与细胞外基质成分结合,包括透明质酸(HA)和骨桥蛋白(OPN),以及与信使分子结合,如肿瘤微环境中的生长因子,它们有助于维持恶性细胞的干性。Nanog和Oct是多能干细胞,在肿瘤干细胞的发育中发挥作用。本研究的目的是研究CD44, Nanog和Oct 4在结直肠癌患者体育场IV中的表达。使用胶原蛋白I提取患者活检,并在37°C下孵育30分钟。活检后,在第3、7和10天用Tali细胞仪和间接免疫染色分析组织中CD44、Nanog和OCT 4的表达。结果与colo细胞株进行了比较。结果显示,来自高加索结肠癌患者的系色细胞样本之间的基因表达存在显著差异。这些差异需要对印度尼西亚结肠癌患者的基因表达进行更深入的研究,这可能对开发未来的抗癌药物有用。CD44是一种细胞粘附分子,被认为是一种癌症标志物,在肿瘤的进展和转移中起着重要作用。CD44在肿瘤发生中的作用是由于它与细胞外基质成分结合,包括透明质酸(HA)和骨桥蛋白(OPN),以及与信使分子结合,如肿瘤微环境中的生长因子,它们有助于维持恶性细胞的干性。Nanog和Oct是多能干细胞,在肿瘤干细胞的发育中发挥作用。本研究的目的是研究CD44, Nanog和Oct 4在结直肠癌患者体育场IV中的表达。使用胶原蛋白I提取患者活检,并在37°C下孵育30分钟。活检后,在第3、7和10天用Tali细胞仪和间接免疫染色分析组织中CD44、Nanog和OCT 4的表达。结果与colo细胞株进行了比较。结果显示,两组间基因表达差异有统计学意义。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Characterization of colon cancer cells from a patient at Ciptomangun Kusumo National Hospital using CD44, NANOG and OCT4 gene
CD44 is a cell adhesion molecule and has been recognized as a cancer marker that plays an important role in tumor progression and metastasis. The role of CD44 in tumorigenesis is due to its binding to extracellular matrix components, including hyaluronan (HA) and osteopontin (OPN) and to messenger molecules, such as growth factors present in the tumor microenvironment where they contribute to the maintenance of the stemness of malignant cells. Nanog and Oct are pluripotent stem cells that play roles in development of cancer stem cell. The aim of this research was to investigate expression of CD44, Nanog and Oct 4 at colorectal patient stadium IV. Biopsy of patient was extracted using collagenese I and incubate at 37°C for 30 minutes. Upon biopsy, the tissues were analyzed using Tali cytometer and indirect immunostaining for CD44, Nanog and OCT 4 expressions at days 3, 7 and 10. The results were compared with that of colo cell line. Results revealed the significance differences in gene expression between samples from patients with line colo cells originating from Caucasian colon cancer patients. Such differences necessitated for more in-depth researches towards the gene expression of Indonesian colon cancer patients that may prove to be useful for developing future anti-cancer drugs.CD44 is a cell adhesion molecule and has been recognized as a cancer marker that plays an important role in tumor progression and metastasis. The role of CD44 in tumorigenesis is due to its binding to extracellular matrix components, including hyaluronan (HA) and osteopontin (OPN) and to messenger molecules, such as growth factors present in the tumor microenvironment where they contribute to the maintenance of the stemness of malignant cells. Nanog and Oct are pluripotent stem cells that play roles in development of cancer stem cell. The aim of this research was to investigate expression of CD44, Nanog and Oct 4 at colorectal patient stadium IV. Biopsy of patient was extracted using collagenese I and incubate at 37°C for 30 minutes. Upon biopsy, the tissues were analyzed using Tali cytometer and indirect immunostaining for CD44, Nanog and OCT 4 expressions at days 3, 7 and 10. The results were compared with that of colo cell line. Results revealed the significance differences in gene expression between s...
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