金黄色葡萄球菌抗菌耐药基因qaca / b、smr的检测与表达

IF 1.1 Q3 AGRICULTURE, MULTIDISCIPLINARY

IRAQI JOURNAL OF AGRICULTURAL SCIENCES Pub Date : 2023-08-29 DOI:10.36103/ijas.v54i4.1808

A. A. Jawad, N. M. Utba

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引用次数: 0

摘要

本研究旨在探讨金黄色葡萄球菌对不同防腐剂的耐药性。本研究表明金黄色葡萄球菌对防腐剂的抗性是由于具有smr基因或qacA/B基因，这些基因与对防腐剂的敏感性降低有关。本研究旨在确定金黄色葡萄球菌对氯二酚耐药菌株的频率，以及这些菌株中上述基因的存在以及氯二酚对这些基因表达的影响。从巴格达皮肤感染中分离的189株临床分离物经显微镜和生化试验鉴定为金黄色葡萄球菌。鉴定了金黄色葡萄球菌对氯二酚的耐药性，并对其进行了氯二酚MIC测定。PCR法检测25株菌株的耐药基因(qacA/B、smr)，结果显示21株(84%)含qacA/B基因。而smr基因未在任何分离株中发现。此外，氯二酚对这些菌株的qacA/B基因表达没有影响。

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ANTISEPTICS RESISTANCE GENES (QACA/B, SMR) DETECTION AND EXPRESSION IN STAPHYLOCOCCUS AUREUS

This study was aimed to investigate the resistance of S. aureus to different antiseptics.This research indicate that the resistance of S.aureus to antiseptics is due to possessing either the smr gene or the qacA/B genes that associated with decreased susceptibility to antiseptics there for this study amid to determine the frequencies of S. aureus chloroxylenol resistant isolates and the presence of the previous genes in these isolates as well as the effect of chloroxylenol on the expression of these genes.189 clinical isolates isolated from skin infections identified as S. aureus in Baghdad by microscopical and biochemical tests. The chloroxylenol resistance S. aureus isolates was identified and chloroxylenol MIC was evaluated for these isolates. Antiseptic resistance genes (qacA/B, smr) were detected by PCR method and the results reveled that 21(84%) out of 25 isolates harbored qacA/B gene. While the smr gene was not demonstrated in any isolates. Furthermore, the chloroxylenol had no effect on qacA/B gene expression in these isolates.

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来源期刊

IRAQI JOURNAL OF AGRICULTURAL SCIENCES AGRICULTURE, MULTIDISCIPLINARY-

CiteScore

2.30

自引率

50.00%

发文量

140

审稿时长

6 weeks

期刊介绍： IRAQI JOURNAL OF AGRICULTURAL SCIENCES (IJAS)is the first agric. scientific and refereed journal established in Iraq. The first volume was published in 1966. IJAS is registered in the number 137 in 1988 of the Baghdad National Library. Years ago, it was published with one issue a year. For the time being, it is published bimonthly (6 issues for a volume). IJAS Deal with: Field Crops. Plant Breeding. Agricultural Economics. Agricultural Extension. Agricultural Mechanization. Basic sciences. Hort. Sciences. Animal Husbandry. Food Technology, Plant Pathology. Plant Entomology. Poultry Sciences. Soil Sciences. Water Resources. Veterinary. Biology.