巨叶Leea总生物碱、单宁、黄酮类、酚类和绿原酸含量的定量测定。Hornem交货

D. SarvadeDattatray
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引用次数: 1

摘要

目的:测定大叶Leea根、茎、叶粗甲醇提取物的总生物碱含量(TAC)、总单宁含量(TTC)、总酚含量(TPC)和总黄酮含量(TFC)。Hornem交货。它是一种重要的药用植物,属于Vitaceae,在民族医学中被广泛使用,具有重要的治疗意义。利用高效薄层色谱法测定植物根、茎、叶中绿原酸的含量。材料与方法:以大叶橐吾根、茎、叶粉为原料制备提取液并定量。用现代分析法测定总生物碱的含量,用滴定法测定总单宁的含量,用氯化铝法测定总黄酮的含量,用Folin-Ciocalteu法测定总酚的含量。采用高效液相色谱法测定绿原酸的含量。结果:根、茎、叶甲醇提取物的TAC分别为0.37%、0.50%、0.52% w/w, TTC分别为2.01%、1.23%、3.67% w/w。根、茎、叶甲醇提取物的TFC和TPC分别为361.67±14.43、233.33±5.77和395±25 mg杨桃素当量/ g和243.33±18.01、232.33±2.31和376±8.54 mg没食子酸当量/ g。茎叶中绿原酸含量分别为27.24和12.21 μg/mg,根中不含绿原酸。结论:这项研究的结果强调了一个强有力的生物碱,单宁、黄酮、酚、绿原酸含量的methanolic提取l、因此可以药用的组件存在于植物具有不同的生物活性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Quantification of total alkaloid, tannin, flavonoid, phenolic, and chlorogenic acid contents of Leea macrophylla Roxb. ex Hornem
Objective: The aim of the present work was to assess the total alkaloid content (TAC), total tannin content (TTC), total phenolic content (TPC), and total flavonoid content (TFC) of crude methanolic extract of root, stem, and leaf of Leea macrophylla Roxb. ex Hornem. which is a medicinally important plant belonging to the family Vitaceae and used extensively in ethnomedicine with its significant therapeutic significance. This article also aims to assess chlorogenic acid content in root, stem, and leaf of plant using high profile thin-layer chromatography (HPTLC). Materials and Methods: Root, stem, and leaf powder of L. macrophylla were used for extract preparation and quantification. The amount of total alkaloids analyzed by modern method of analysis, total tannin by titrimetric method, the amount of total flavonoids by aluminum chloride assay, and total phenols by Folin–Ciocalteu assay. HPTLC method has been used for quantification of chlorogenic acid. Results: The TAC of the methanolic extract of root, stem, and leaf was 0.37%, 0.50%, and 0.52% w/w, respectively, TTC was 2.01%, 1.23%, and 3.67% w/w, respectively. The TFC and TPC of the methanolic extract of root, stem, and leaf were 361.67 ± 14.43, 233.33 ± 5.77, and 395 ± 25 mg chrysin equivalent per gram and 243.33 ± 18.01, 232.33 ± 2.31, and 376 ± 8.54 mg gallic acid equivalent per gram, respectively. Chlorogenic acid contents of stem and leaf are 27.24 and 12.21 μg/mg, while root does not show its presence using this method. Conclusion: The results of the study highlighted a potent alkaloid, tannins, flavonoid, phenol, and chlorogenic acid contents in the methanolic extract of L. macrophylla and thus can be used for medicinal purposes as components present in the plant possesses varied biological activities.
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