苍叶莲离体再生的研究。(兰科)

E. S. Gladies, T. Krishnaveni, B. S. C. Devi
{"title":"苍叶莲离体再生的研究。(兰科)","authors":"E. S. Gladies, T. Krishnaveni, B. S. C. Devi","doi":"10.5958/2349-2104.2019.00003.2","DOIUrl":null,"url":null,"abstract":"Orchids exhibit a wide range of diversity in forms, size, colour and texture of flowers beyond the imagination of human mind. Orchids grow in nature through seeds but in the absence of appropriate hosts, they do not germinate in adequate number. This obstacle was overcome by adapting tissue culture technique for appropriate germination of orchids. In vitro techniques have been successfully carried out for the mass propagation of orchid plantlets. Hence, a preliminary study was carried out to develop a suitable protocol for mass multiplication of Pholidota pallida. MS (Murashige and Skoog) medium was found to be suitable for the asymbiotic seed germination of Pholidota pallida. MS medium supplemented with IAA (Indole-3-acetic acid), IBA (Indole-3butyric acid), BAP (6-Benzylaminopurine) and KIN (Kinetin) individually and in combinations induced direct protocorm like bodies. Hormone-free MS basal medium was found suitable for the conversion of PLBs (protocorm-like bodies) into complete plantlets","PeriodicalId":7285,"journal":{"name":"Advances in Applied Science Research","volume":"99 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"In vitro regeneration of Pholidota pallida Lindl. (Orchidaceae)\",\"authors\":\"E. S. Gladies, T. Krishnaveni, B. S. C. Devi\",\"doi\":\"10.5958/2349-2104.2019.00003.2\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Orchids exhibit a wide range of diversity in forms, size, colour and texture of flowers beyond the imagination of human mind. Orchids grow in nature through seeds but in the absence of appropriate hosts, they do not germinate in adequate number. This obstacle was overcome by adapting tissue culture technique for appropriate germination of orchids. In vitro techniques have been successfully carried out for the mass propagation of orchid plantlets. Hence, a preliminary study was carried out to develop a suitable protocol for mass multiplication of Pholidota pallida. MS (Murashige and Skoog) medium was found to be suitable for the asymbiotic seed germination of Pholidota pallida. MS medium supplemented with IAA (Indole-3-acetic acid), IBA (Indole-3butyric acid), BAP (6-Benzylaminopurine) and KIN (Kinetin) individually and in combinations induced direct protocorm like bodies. Hormone-free MS basal medium was found suitable for the conversion of PLBs (protocorm-like bodies) into complete plantlets\",\"PeriodicalId\":7285,\"journal\":{\"name\":\"Advances in Applied Science Research\",\"volume\":\"99 1\",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2019-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Advances in Applied Science Research\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.5958/2349-2104.2019.00003.2\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Advances in Applied Science Research","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.5958/2349-2104.2019.00003.2","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

摘要

兰花在形态、大小、颜色和质地上的多样性超出了人类的想象。兰花在自然界中通过种子生长,但由于没有合适的寄主,它们不能大量发芽。采用组织培养技术使兰花适宜发芽,克服了这一障碍。已经成功地进行了兰花植株的离体繁殖技术。因此,为制定一套适宜的白藻大规模繁殖方案进行了初步研究。MS (Murashige和Skoog)培养基适合于白花百合种子的非共生萌发。分别添加IAA(吲哚-3-乙酸)、IBA(吲哚-3丁酸)、BAP(6-苄基氨基嘌呤)和KIN (Kinetin)的MS培养基可诱导直接原球样体。发现无激素MS基础培养基适合将PLBs(原球茎样体)转化为完整植株
本文章由计算机程序翻译,如有差异,请以英文原文为准。
In vitro regeneration of Pholidota pallida Lindl. (Orchidaceae)
Orchids exhibit a wide range of diversity in forms, size, colour and texture of flowers beyond the imagination of human mind. Orchids grow in nature through seeds but in the absence of appropriate hosts, they do not germinate in adequate number. This obstacle was overcome by adapting tissue culture technique for appropriate germination of orchids. In vitro techniques have been successfully carried out for the mass propagation of orchid plantlets. Hence, a preliminary study was carried out to develop a suitable protocol for mass multiplication of Pholidota pallida. MS (Murashige and Skoog) medium was found to be suitable for the asymbiotic seed germination of Pholidota pallida. MS medium supplemented with IAA (Indole-3-acetic acid), IBA (Indole-3butyric acid), BAP (6-Benzylaminopurine) and KIN (Kinetin) individually and in combinations induced direct protocorm like bodies. Hormone-free MS basal medium was found suitable for the conversion of PLBs (protocorm-like bodies) into complete plantlets
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信