不同生长阶段小球藻的抗氧化反应

Q3 Environmental Science
N. Yusuf, Nur Maisarah Athirah, Suhaila A
{"title":"不同生长阶段小球藻的抗氧化反应","authors":"N. Yusuf, Nur Maisarah Athirah, Suhaila A","doi":"10.15578/squalen.692","DOIUrl":null,"url":null,"abstract":"Chlorella vulqaris is a unicellular microorganism that offers health benefits due to its concentrated antioxidant production. This microalga has received huge attention due to its natural antioxidative property as an alternative antioxidant source because of its rapid growth, easy and flexible culture. Research to date only focuses on the growth and antioxidant production in a selected growth phase, especially exponential and stationary phases; however, so far, limited reports on the production of antioxidants in all growth phases of C. vulgaris. Thus, this study determines the growth, the enzymatic (Catalase, CAT; Ascorbate Peroxidase, APX; and guaiacol peroxidase, gPOD) specific activities and the amount of the non-enzymatic antioxidants (a-tocopherol, ascorbic acid and carotenoids) of C. vulgaris in five growth phases. Chlorella vulgaris was cultured in F/2 medium at 25±2 °C under laboratory conditions. CAT specific activities were the highest at the exponential phase (1.50±0.08 units/mg protein), whereas APX and gPOD were induced at the lag phases of 37.13±4.93 units/mg protein and 1.31±0.03 units/mg protein, respectively. The amount of a-tocopherol was accumulated at the stationary phase (97.3±4.18 µg/g.fwt), whereas the highest amount of ascorbic acid (266.67±22.22 µg/g.fwt) and carotenoids (8.16±2.52 µg/g.fwt) were at the decline phase. Production of enzymatic and non-enzymatic antioxidants in the microalgae cells indicated that they efficiently scavenged reactive oxygen species (ROS) and converted them into less harmful substances. In addition, the production of these antioxidants in different growth phases can be used as a guideline to produce massive antioxidants, which can be commercialized in the food and pharmaceutical industries.","PeriodicalId":21935,"journal":{"name":"Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2022-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":"{\"title\":\"Antioxidative Responses of Chlorella vulgaris Under Different Growth Phases\",\"authors\":\"N. Yusuf, Nur Maisarah Athirah, Suhaila A\",\"doi\":\"10.15578/squalen.692\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Chlorella vulqaris is a unicellular microorganism that offers health benefits due to its concentrated antioxidant production. This microalga has received huge attention due to its natural antioxidative property as an alternative antioxidant source because of its rapid growth, easy and flexible culture. Research to date only focuses on the growth and antioxidant production in a selected growth phase, especially exponential and stationary phases; however, so far, limited reports on the production of antioxidants in all growth phases of C. vulgaris. Thus, this study determines the growth, the enzymatic (Catalase, CAT; Ascorbate Peroxidase, APX; and guaiacol peroxidase, gPOD) specific activities and the amount of the non-enzymatic antioxidants (a-tocopherol, ascorbic acid and carotenoids) of C. vulgaris in five growth phases. Chlorella vulgaris was cultured in F/2 medium at 25±2 °C under laboratory conditions. CAT specific activities were the highest at the exponential phase (1.50±0.08 units/mg protein), whereas APX and gPOD were induced at the lag phases of 37.13±4.93 units/mg protein and 1.31±0.03 units/mg protein, respectively. The amount of a-tocopherol was accumulated at the stationary phase (97.3±4.18 µg/g.fwt), whereas the highest amount of ascorbic acid (266.67±22.22 µg/g.fwt) and carotenoids (8.16±2.52 µg/g.fwt) were at the decline phase. Production of enzymatic and non-enzymatic antioxidants in the microalgae cells indicated that they efficiently scavenged reactive oxygen species (ROS) and converted them into less harmful substances. In addition, the production of these antioxidants in different growth phases can be used as a guideline to produce massive antioxidants, which can be commercialized in the food and pharmaceutical industries.\",\"PeriodicalId\":21935,\"journal\":{\"name\":\"Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2022-12-30\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.15578/squalen.692\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"Environmental Science\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.15578/squalen.692","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"Environmental Science","Score":null,"Total":0}
引用次数: 1

摘要

小球藻是一种单细胞微生物,由于其浓缩的抗氧化剂生产而对健康有益。该微藻因其生长迅速、培养容易、灵活等特点,具有天然的抗氧化特性,作为一种可替代的抗氧化来源而受到广泛关注。迄今为止的研究只关注特定生长阶段的生长和抗氧化剂的产生,特别是指数和平稳阶段;然而,迄今为止,关于芥蓝各生长阶段抗氧化剂产生的报道有限。因此,本研究确定了生长过程中,酶(过氧化氢酶,CAT;抗坏血酸过氧化物酶;不同生长时期愈创木酚过氧化物酶(gPOD)比活性和非酶抗氧化剂(a-生育酚、抗坏血酸和类胡萝卜素)含量的变化。实验条件下,在25±2℃的F/2培养基中培养普通小球藻。CAT的特异活性在指数期最高(1.50±0.08单位/mg蛋白),而APX和gPOD的特异活性在滞后期分别为37.13±4.93单位/mg蛋白和1.31±0.03单位/mg蛋白。a-生育酚含量在稳定期积累(97.3±4.18µg/g.fwt),抗坏血酸和类胡萝卜素含量在下降期积累(266.67±22.22µg/g.fwt),类胡萝卜素含量为8.16±2.52µg/g.fwt。微藻细胞中酶促和非酶促抗氧化剂的产生表明它们有效地清除活性氧(ROS)并将其转化为危害较小的物质。此外,这些抗氧化剂在不同生长阶段的生产可以作为生产大量抗氧化剂的指导方针,这些抗氧化剂可以在食品和制药工业中商业化。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Antioxidative Responses of Chlorella vulgaris Under Different Growth Phases
Chlorella vulqaris is a unicellular microorganism that offers health benefits due to its concentrated antioxidant production. This microalga has received huge attention due to its natural antioxidative property as an alternative antioxidant source because of its rapid growth, easy and flexible culture. Research to date only focuses on the growth and antioxidant production in a selected growth phase, especially exponential and stationary phases; however, so far, limited reports on the production of antioxidants in all growth phases of C. vulgaris. Thus, this study determines the growth, the enzymatic (Catalase, CAT; Ascorbate Peroxidase, APX; and guaiacol peroxidase, gPOD) specific activities and the amount of the non-enzymatic antioxidants (a-tocopherol, ascorbic acid and carotenoids) of C. vulgaris in five growth phases. Chlorella vulgaris was cultured in F/2 medium at 25±2 °C under laboratory conditions. CAT specific activities were the highest at the exponential phase (1.50±0.08 units/mg protein), whereas APX and gPOD were induced at the lag phases of 37.13±4.93 units/mg protein and 1.31±0.03 units/mg protein, respectively. The amount of a-tocopherol was accumulated at the stationary phase (97.3±4.18 µg/g.fwt), whereas the highest amount of ascorbic acid (266.67±22.22 µg/g.fwt) and carotenoids (8.16±2.52 µg/g.fwt) were at the decline phase. Production of enzymatic and non-enzymatic antioxidants in the microalgae cells indicated that they efficiently scavenged reactive oxygen species (ROS) and converted them into less harmful substances. In addition, the production of these antioxidants in different growth phases can be used as a guideline to produce massive antioxidants, which can be commercialized in the food and pharmaceutical industries.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
CiteScore
1.40
自引率
0.00%
发文量
10
审稿时长
16 weeks
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信