光刺激对乳酸菌培养产量及乳杆菌素活性影响的研究

Q Engineering
S. Tikhonov, N. Tikhonova, I. G. Pestova, M. S. Tikhonova
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引用次数: 0

摘要

本文报道了乳杆菌接种物的光刺激对培养泡腾率和所得乳酸链球菌素活性影响的研究结果。研究人员使用冷冻乳酸杆菌接种物来生产乳酸链球菌素。他们在5.0 ml无菌MRS管中激活培养物,在37°C的培养箱中保存48小时。孵育前,用波长430 ~ 470 nm、光通量强度35 MW/cm2的蓝光处理乳酸乳杆菌培养物1小时(实验组)。对照组的细菌样本不进行光照处理。一个人用MRS培养基进行发酵。为了确定培养滴度,作者在MRS培养基上进行了播种。研究人员通过在琼脂培养基上用5mmol过氧化氢培养乳酸乳杆菌,确定了氧化应激对乳酸乳杆菌稳定性的影响。为了在播种前鉴定其滴度和抗氧化能力,对培养物进行了具有特定特性和处理时间的蓝光处理。细菌培养24小时后,与对照组相比,可使nisin活性提高19.3%,或提高558 IU/ml。研究人员证明,在培养前用蓝色光谱光进行初步照射的背景下,乳酸乳杆菌培养物的滴度比对照组样品高56.3%。蓝光处理乳酸乳杆菌培养物可使其在氧化胁迫下的生存能力提高75%。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Research of the Photostimulation Effect on the Lactobacillus Lactis Culture Productivity and Resulting Nisin Activity
The article concerns the study results of the photostimulation influence of the Lactobacillus lactis inoculum on the cultural effervescence and obtained nisin activity. The researchers used frozen Lactobacillus lactis inoculum to produce nisin. They activated the culture in a 5.0 ml sterile MRS tube kept in an incubator at 37 °C for 48 hours. Before incubation, Lactobacillus lactis culture was treated with blue spectrum light with a wavelength of 430–470 nm and a luminous flux intensity of 35 MW/cm2 for 1 hour (experimental group). Bacterial samples of the control group were not treated with light. A man used the MRS medium for fermentation. To determine the culture titer, the authors run sowing on the MRS medium. The researchers defined an oxidative stress influence on the Lactobacillus lactis stability by cultivation on an agar medium using 5 mmol of hydrogen peroxide. To identify the titer and resistance to oxidative stress before sowing, the cultures were treated with blue light with the specified characteristics and processing time. It enables to increase the nisin activity after 24 hours of bacterial cultivation compared to the control group by 19.3%, or by 558 IU/ml. The researchers proved that the titer of Lactobacillus lactis culture against the background of preliminary irradiation with blue spectrum light before cultivation is 56.3% higher than the samples of the control group. Treatment of Lactobacillus lactis culture with blue spectrum light increases its viability under oxidative stress by 75%.
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来源期刊
Agro Food Industry Hi-tech
Agro Food Industry Hi-tech 工程技术-生物工程与应用微生物
CiteScore
0.29
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0.00%
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0
审稿时长
6-12 weeks
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