Qualitative evaluation of slaughterhouse bovine ovaries by histological study

This study was conducted at the Anatomy and Physiology Laboratory, Faculty of Sustainable Agriculture, Universiti Malaysia Sabah Sandakan Campus from August 2016 until October 2016 to do a reach work on the analysis of the slaughterhouse bovine ovaries in term of the quality by histochemistry studies. The objectives that been set for this study were to identify the quality of follicles in bovine ovaries and to find out the relationship between the quality of follicles and ovarian state. The ovaries were collected from slaughterhouse and morphological evaluations such as weight and number of visible follicles on the surface of the ovaries were counted. The ovaries been classified into two groups as ovary with corpus luteum (CL+) and without corpus luteum (CL-). The recorded percentages of ovaries that been retrieved from the slaughterhouse is 75.0% CL- and 25.0% CL+. The visible follicles on the surface of the ovaries that been recorded at average level for CL+ (23.0 ± 2.4) is lower than the CL­(26.0 ± 2.2). From the collected ovaries represented several ovaries from both groups been used for histochemistry study. The ovaries then been prepared for histological study by fixation, dehydration, clearing, embedding, and blocking with paraffin wax. The waxed sample block been trimmed with microtome at 6µm and stained with Hematoxylene and Eosin (HE). The sample observed under microscope and the number of follicles and the granulosa cells integrity been recorded. From the finding, the CL- ovaries were contained good quality follicles (8.8 ± 0.3) at grade