人囊性包虫病:通过间接血凝试验、酶联免疫吸附试验、免疫电泳和免疫印迹对手术确诊患者的血清学诊断与通过成像技术诊断的病例

IF 5 Q3 Medicine
H. Zait, B. Hamrioui
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引用次数: 7

摘要

人类囊性包虫病(CE)的诊断是基于影像学表现和血清学。血清学是第一线检查,其次是影像学检查。材料与方法本研究共收集了268份CE患者血清样本。采用间接血凝试验(IHA)、酶联免疫吸附试验(ELISA)、免疫印迹试验(IB)和免疫电泳(IEP)同时进行血清诊断。结果在手术确诊的CE患者血清样本中,IHA和IgG-ELISA阳性率分别为83.7%(77.2% ~ 89.0%)和80.7%(73.9% ~ 86.4%)。IgG-IB的阳性率高于IEP,分别为81.3%[74.6% ~ 86.9%]和62.0%[54.2% ~ 69.5%]。IHA和ELISA结果采用受试者工作曲线产生的截止值进行分析。IHA (cut-off≥1/128)和ELISA (>1.16指数)。有提示性影像学表现和IB棘球蚴阳性的患者对IHA、IgG-ELISA和IEP的敏感性分别为86.2%[78.0%-92.2%]、72.5%[62.8%-80.9%]、49.0%[39.0%-59.1%]。在确诊的CE组中,所有测试结果均为假阴性。总体而言,18.6%的阴性结果为IgG-IB检测。在所有试验中均观察到与非包虫血清样品的交叉反应。只有1例携带带绦虫血清的患者通过IB与8/12 kDa带交叉反应,我们观察到IHA、IgG-ELISA、IEP和IgG-IB的特异性分别为73%[63.2% ~ 81.4%]、87%[78.8% ~ 92.9%]、99%[94.6% ~ 100.0%]和99%[94.6% ~ 100.0%]。血清学对肺囊肿的敏感性较低(74%)。肝囊肿的敏感性更好,尤其是IgG-IB(96%)。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Human cystic echinococcosis: Serological diagnosis by indirect hemagglutination test, enzyme-linked immunosorbent assay, immunoelectrophoresis, and immunoblotting in surgically confirmed patients versus cases diagnosed by imaging techniques

Introduction

Human cystic echinococcosis (CE) diagnosis is based on imaging findings and serology. Serology is the first-line test followed by imaging tests.

Materials and methods

A total of 268 serum samples from CE patients were included in this study. The serodiagnosis was made simultaneously by indirect hemagglutination assay (IHA), enzyme-linked immunosorbent assay (ELISA), immunoblotting test (IB), and immunoelectrophoresis (IEP).

Results

In serum samples belonging to surgically confirmed CE patients, we observed a percentage of positivity of 83.7% [77.2%–89.0%] and 80.7% [73.9%–86.4%] for IHA and IgG-ELISA, respectively. IgG-IB was associated with a higher positivity rate than IEP with 81.3% [74.6%–86.9%] and 62.0% [54.2%–69.5%], respectively. IHA and ELISA results were analyzed using cut-off generated by receiver operating curves. The best diagnostic performances were achieved by IHA (cut-off ≥ 1/128) and ELISA (> 1.16 index). Sensitivities reported in patients with suggestive imaging findings and positive Echinococcus IB were 86.2% [78.0%–92.2%], 72.5% [62.8%–80.9%], 49.0% [39.0%–59.1%] for IHA, IgG-ELISA, and IEP, respectively. All tests gave false negative results in the confirmed CE group. Overall, 18.6% of negative results were obtained by IgG-IB test. Cross-reactivities with non-hydatid serum samples were observed in all tests. Only one patient carrying Taenia saginata serum cross-reacted with 8/12 kDa band by IB. We observed specificity at 73% [63.2%–81.4%], 87% [78.8%–92.9%], 99% [94.6%–100.0%], and 99% [94.6%–100.0%] with IHA, IgG-ELISA, IEP, and IgG-IB, respectively. Serology was less sensitive (74%) in lung cysts. Sensitivity was better in liver cysts, especially by IgG-IB (96%).

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来源期刊
Medecine et maladies infectieuses
Medecine et maladies infectieuses 医学-传染病学
CiteScore
1.10
自引率
0.00%
发文量
0
审稿时长
10.7 weeks
期刊介绍: L''organe d''expression de la Société de Pathologie Infectieuse de Langue Française (SPILF). Médecine et Maladies Infectieuses is the official publication of the Société de Pathologie Infectieuse de Langue Française (SPILF). Médecine et Maladies Infectieuses is indexed in the major databases: Medline, Web of Science/Clarivate and Scopus. The journal publishes scientific /research articles, general reviews, short communications and letters, in both English and French. The journal welcomes submissions on the various aspects of infectious pathologies and pathogenic agents. Médecine et Maladies Infectieuses focuses on clinical therapeutics, nosocomial infections, biology, prevention, as well as epidemiology and therapeutics.
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