抗氧化物活性、类黄酮和珍珠壳总表型(Pinctada maxima)

Khoirul Ngibad, Afidatul Muadifah, Datin An Nisa Sukmawati
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引用次数: 0

摘要

抗氧化化合物能够抑制或抵御自由基。包括珍珠贝在内的生物可以产生次生代谢物化合物(酚类物质、生物碱和萜类物质)。珠蚌壳的抗氧化能力、类黄酮含量和总酚类物质的研究尚未见报道。本研究旨在采用DPPH法测定珠蚌壳乙醇提取物(EECKM)的体外抗氧化能力。此外,还旨在测定EECKM的总黄酮和酚类含量。用乙醇浸泡珍珠贻贝粉,浓缩滤液,得到浓缩提取物。用紫外-可见分光光度计测定EECKM中的类黄酮和酚类含量。DPPH法测定抗氧化活性。EECKM的产率为1.73%,黄酮含量为0 mg QE/g,总酚含量为4.8 mg GAE/g。当浓度为100 mg / L时,其抗氧化活性最高,抑制DPPH自由基的百分比值为67.1%。
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Aktivitas Antioksidan, Kadar Flavonoid, dan Fenolik Total Cangkang Kerang Mutiara (Pinctada maxima)
Antioxidant compounds are able to dampen or ward off free radicals. Living things including pearl mussels (Pinctada maxima) can produce secondary metabolite compounds (phenolics, alkaloids, and terpenoids). There have been no studies on the antioxidant potential, flavonoid content, and total phenolics of pearl clam shells. This study aimed to determine the antioxidant potential of pearl clam shell ethanol extract (EECKM) in vitro using the DPPH method. In addition, it aims to determine the total flavonoid and phenolic levels of EECKM. The powder of pearl mussel shells is soaked with ethanol and the filtrate is concentrated until a concentrated extract is obtained. Flavonoid and phenolic levels from EECKM were measured using a UV-Vis spectrophotometer. Measurement of antioxidant activity using the DPPH method. EECKM has a yield of 1.73%, flavonoid levels of 0 mg QE/g, and total phenolics of 4.8 mg GAE/g. The extract has the highest antioxidant activity for a concentration of 100 mg / L with a percent value of DPPH radical inhibition of 67.1%.
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