基于微通道阵列的细胞膜光动力穿孔技术

2007 IEEE 20th International Conference on Micro Electro Mechanical Systems (MEMS) Pub Date : 2007-12-01 DOI:10.1109/MEMSYS.2007.4433067

K. Iso, T. Saito, H. Muguruma, H. Tabata, S. Konishi

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引用次数: 2

摘要

本文报道了一种面向胞内技术的基于微通道阵列(MCA)的光动力细胞穿孔器的设计、制造和实验结果。该穿孔器将用于改进全细胞膜片钳技术。穿孔器由平面微通道和中动脉电极组成。射孔器利用微通道进行细胞吸入和化学供应。微通道的直径为ph2 μ m。将光敏剂(PS)通过微通道引入细胞膜，激活后的光敏剂在细胞膜上进行光动力穿孔，利用直径小至数十微米的PC12细胞成功实现了细胞膜的光动力穿孔。

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Photodynamic perforation of cell membrane on Micro Channel Array toward intracellular technology

This paper reports the design, fabrication, and experimental results for a micro channel array (MCA)-based photodynamic cell perforator toward intracellular technology. The perforator will be developed to improve whole-cell patch-clamp technique. The perforator consists of planar micro channels and electrodes of the MCA. The perforator utilizes the micro channel for both cell suction and chemical supply. The diameter of the micro channel is Phi2 mum. The photosensitizer (PS) is introduced to the cell membrane through the micro channel, and then the cell membrane is perforated photodynamically by activated PS. Photodynamic perforation of the cell membrane performed successfully in the experiment using PC12 cells with diameters as small as tens of mum.

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来源期刊

2007 IEEE 20th International Conference on Micro Electro Mechanical Systems (MEMS)

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