Maick Meneguzzo Prado, D. J. Kovalski, W. B. Torrez, B. Bueno-Silva, M. Feres, J. de Almeida, L. Porto
{"title":"搅拌生物反应器中多菌种牙周生物膜的表征及生长动力学","authors":"Maick Meneguzzo Prado, D. J. Kovalski, W. B. Torrez, B. Bueno-Silva, M. Feres, J. de Almeida, L. Porto","doi":"10.1166/asem.2020.2687","DOIUrl":null,"url":null,"abstract":"This study aimed to validate an in vitro periodontal biofilm model with complex subgingival microbial communities grown in a stirred bioreactor. Experimental biofilms were characterized and examined for their growth kinetics over time. After incubation under anaerobic conditions\n for 3 days, biofilm cultures formed on the substrate were collected and analyzed. The specific growth rate in the exponential phase was estimated to be 0.3915 h−1, with no significant differences in growth kinetics and CFU counts between the replicates (P > 0.05).\n The biofilm architecture was consistent in each time-point, with bacterial cells presenting different morphology and completely colonizing the cementum surface. To conclude, the three-day subgingival biofilm model developed in our study successfully mimicked the growth of complex microbial\n communities.","PeriodicalId":7213,"journal":{"name":"Advanced Science, Engineering and Medicine","volume":"1 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2020-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Characterization and Growth Kinetics of a Multispecies Periodontal Biofilm Developed in a Stirred Bioreactor\",\"authors\":\"Maick Meneguzzo Prado, D. J. Kovalski, W. B. Torrez, B. Bueno-Silva, M. Feres, J. de Almeida, L. Porto\",\"doi\":\"10.1166/asem.2020.2687\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"This study aimed to validate an in vitro periodontal biofilm model with complex subgingival microbial communities grown in a stirred bioreactor. Experimental biofilms were characterized and examined for their growth kinetics over time. After incubation under anaerobic conditions\\n for 3 days, biofilm cultures formed on the substrate were collected and analyzed. The specific growth rate in the exponential phase was estimated to be 0.3915 h−1, with no significant differences in growth kinetics and CFU counts between the replicates (P > 0.05).\\n The biofilm architecture was consistent in each time-point, with bacterial cells presenting different morphology and completely colonizing the cementum surface. To conclude, the three-day subgingival biofilm model developed in our study successfully mimicked the growth of complex microbial\\n communities.\",\"PeriodicalId\":7213,\"journal\":{\"name\":\"Advanced Science, Engineering and Medicine\",\"volume\":\"1 1\",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2020-11-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Advanced Science, Engineering and Medicine\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1166/asem.2020.2687\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Advanced Science, Engineering and Medicine","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1166/asem.2020.2687","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Characterization and Growth Kinetics of a Multispecies Periodontal Biofilm Developed in a Stirred Bioreactor
This study aimed to validate an in vitro periodontal biofilm model with complex subgingival microbial communities grown in a stirred bioreactor. Experimental biofilms were characterized and examined for their growth kinetics over time. After incubation under anaerobic conditions
for 3 days, biofilm cultures formed on the substrate were collected and analyzed. The specific growth rate in the exponential phase was estimated to be 0.3915 h−1, with no significant differences in growth kinetics and CFU counts between the replicates (P > 0.05).
The biofilm architecture was consistent in each time-point, with bacterial cells presenting different morphology and completely colonizing the cementum surface. To conclude, the three-day subgingival biofilm model developed in our study successfully mimicked the growth of complex microbial
communities.