火鸡血清和分泌物中免疫球蛋白IgA、IgM和IgG的定量测定

J. Goudswaard , A. Noordzij, R.H. van Dam, J.A. van der Donk
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引用次数: 11

摘要

测定了个体血清和混合血清中免疫球蛋白IgA、IgM和IgG的含量。正常健康鸟类Ig水平的变异性相当高:IgA:平均值0.633 mg/ml (4.0 x-2.5 x);IgM:平均值4.37 mg/ml (0.5 ~ 1.4 x), IgG:平均值8.92 mg/ml (0.6 ~ 1.7 x),测定蛋黄、蛋清、胆汁及部分眼内组织免疫球蛋白水平。一个有趣的发现是,到目前为止,在20个蛋黄样本中,上述变异对IgG水平的影响并不大:平均值为5.1 mg/ml (0.86 x-1.17 x)。尽管在火鸡胆汁中可以检测到IgG和IgM,但IgA在这种分泌物中占主导地位。在房水、虹膜组织和玻璃体中只能检测到IgG。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Quantification of Turkey Immunoglobulins IgA, IgM and IgG in Serum and Secretions

The immunoglobulins IgA, IgM and IgG of the turkey were quantitated in individual serum samples as well as in pooled sera. The variability of Ig levels in normal, healthy birds was quite high: IgA: mean value 0.633 mg/ml (4.0 x-2.5 x); IgM: mean value 4.37 mg/ml (0.5 x-1.4 x) and IgG: mean value 8.92 mg/ml (0.6 x-1.7 x).

Immunoglobulin levels in egg-yolk, egg-white, bile and some intraocular tissues were quantitated as well. An interesting finding was, that the forementioned variability was by far not so high with respect to IgG levels in 20 egg-yolk samples: mean value 5.1 mg/ml (0.86 x-1.17 x). Though IgG and IgM could be detected in pooled turkey bile, IgA predominated in this secretion. In aqueous humor, iris tissue and vitreous body only IgG could be detected.

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