在发酵条件下引入乳糖促进剂

Acta Biotechnologica Pub Date : 1991-01-01 DOI:10.1002/ABIO.370110107

P. Neubauer, C. Wolff, M. Hecker, K. Hofmann, L. Meyer, P. Kruschke, H. Heinrich

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引用次数: 17

摘要

使用昂贵的IPTG来诱导tac启动子在工业规模上是不可行的，因为诱导所需的量很大。我们开发了一个用乳糖代替IPTG作为诱导剂诱导tac启动子的系统，其诱导效率相同。在大肠杆菌CW3011 (pOF1.0)中，即使在葡萄糖存在的情况下，乳糖也被用作诱导剂和碳源，可能是由于渗透酶过量产生的结果。

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Introduction of the tac-promoter by lactose under fermentation conditions

The use of the expensive IPTG for induction of the tac-promoter is not practicable at the industrial scale because of the large amounts necessary for induction. We developed a system in which IPTG as inducer was replaced by lactose which induces the tac-promoter with the same efficiency. In E. coli CW3011 (pOF1.0) lactose is utilized as inducer and carbon source even in the presence of glucose, presumably as a consequence of the overproduction of lac-permease.

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来源期刊

Acta Biotechnologica

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