在护理点进行数字核酸诊断的技术平台

IF 0.1 Q4 OTORHINOLARYNGOLOGY
Nadine Borst, Friedrich Schuler, S. Wadle, M. Schulz, M. Specht, Jia Li, Lisa Becherer, Martin Trotter, A. B. Rodríguez-Martínez, N. Paust, R. Zengerle, F. Stetten
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引用次数: 4

摘要

数字放大和离心微流控技术的结合可以实现在护理点(PoC)进行定量和快速诊断。新的离心步乳化单元操作允许高通量液滴生成。不同的数字核酸分析方法,包括PCR、重组酶聚合酶扩增(RPA)和环介导等温扩增(LAMP),已经得到证实。本文介绍了一种新型的综合样本到答案分析方法,并举例说明了HIV的检测和耐药细菌的单细胞分析。在这些基于LabDisk的系统旁边,一种称为DropChip的微流控盒允许仅使用市售实验室设备进行数字放大。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
A technology platform for digital nucleic acid diagnostics at the point of care
Abstract The combination of digital amplification and centrifugal microfluidics can enable quantitative and fast diagnostics at the point of care (PoC). The new unit operation of centrifugal step emulsification allows high throughput droplet generation. Different methods for digital nucleic acid analysis, including PCR, recombinase polymerase amplification (RPA) and loop mediated isothermal amplification (LAMP), have already been demonstrated. Our novel approach of integrated sample-to-answer analysis is introduced, and examples for the detection of HIV and single cell analysis of antibiotic resistant bacteria are presented. Next to these LabDisk based systems, a microfluidic cartridge termed DropChip allows for digital amplification using only commercially available laboratory devices.
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来源期刊
CiteScore
0.80
自引率
0.00%
发文量
1
审稿时长
>12 weeks
期刊介绍: Information not localized
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