BAMLET与5-氟尿嘧啶靶向WNT/& β在结直肠癌细胞中的作用比较连环蛋白通路

IF 0.4 Q4 ONCOLOGY

International Journal of Cancer Management Pub Date : 2022-09-20 DOI:10.5812/ijcm-123140

Marziyeh Babazadeh, Mozhdeh Zamani, P. Mehrbod, P. Mokarram

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引用次数: 1

摘要

背景:WNT/β-catenin信号通路的异常激活涉及多种类型的癌症，特别是结直肠癌(CRC)，这是一种常见的恶性肿瘤。靶向Wnt信号通路作为一种有吸引力的治疗策略已经获得了声誉，主要是因为它具有调节细胞增殖、迁移、分化、血管生成和凋亡的潜力。目的:本研究旨在探讨5-氟尿嘧啶(5-FU)和牛α-乳清蛋白(BAMLET)(油酸与牛α-乳清蛋白的复合物)对结肠癌细胞Wnt信号通路的影响。方法:为此，采用5-FU和BAMLET处理HT-29和HCT116细胞，采用实时荧光定量pcr (RT-qPCR)和Western Blot分析Wnt信号相关蛋白(β-catenin和E-cadherin)和VEGF作为血管生成调节因子的表达水平。结果:与5-FU相比，BAMLET能显著下调β-catenin的表达，上调E-cadherin的表达(P < 0.0001)。处理细胞中VEGF mRNA水平的降低表明5-FU和BAMLET对血管生成的作用。结论:牛α -乳白蛋白(BAMLET)可考虑靶向Wnt信号通路和血管生成。在结直肠癌治疗的发展中，这是值得进一步研究的。

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The Comparative Role of BAMLET and 5-Fluorouracil in Colorectal Cancer Cells by Targeting WNT/& Beta; -Catenin Pathway

Background: Aberrant activation of the WNT/β-catenin signaling pathway is involved in various types of cancers, particularly colorectal cancer (CRC), which is a prevalent malignancy. Targeting the Wnt signaling pathway has gained a reputation as an attractive therapeutic strategy, mainly because of its potential for regulating cell proliferation, migration, differentiation, angiogenesis, and apoptosis. Objectives: The aim of the current research was to investigate the effects of 5-Fluorouracil (5-FU) and bovine alpha-lactalbumin made lethal to tumor cells (BAMLET), a complex of oleic acid with bovine α-lactalbumin protein, on colon cancer cells focusing on the Wnt signaling pathway. Methods: For this purpose, HT-29 and HCT116 cells were treated with 5-FU and BAMLET, and the expression levels of Wnt signaling-related proteins (β-catenin and E-cadherin) and VEGF as angiogenesis regulators were evaluated by quantitative real-time polymerase chain reaction (RT-qPCR) and Western Blot analysis. Results: Bovine alpha-lactalbumin made lethal to tumor cells (BAMLET) treatment down-regulated the expression of β-catenin and up-regulated the expression of E-cadherin significantly compared to the 5-FU (P < 0.0001). The reduced mRNA levels of VEGF in treated cells revealed the effectiveness of 5-FU and BAMLET on angiogenesis. Conclusions: Bovine alpha-lactalbumin made lethal to tumor cells (BAMLET) can be considered for targeting the Wnt signaling pathway and angiogenesis. It is amenable to further investigation in the development of CRC treatment.

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来源期刊

International Journal of Cancer Management ONCOLOGY-

CiteScore

1.10

自引率

0.00%

发文量

期刊介绍： International Journal of Cancer Management (IJCM) publishes peer-reviewed original studies and reviews on cancer etiology, epidemiology and risk factors, novel approach to cancer management including prevention, diagnosis, surgery, radiotherapy, medical oncology, and issues regarding cancer survivorship and palliative care. The scope spans the spectrum of cancer research from the laboratory to the clinic, with special emphasis on translational cancer research that bridge the laboratory and clinic. We also consider original case reports that expand clinical cancer knowledge and convey important best practice messages.