动态荧光光谱法研究高静水压力对蛋白质和脂质膜的影响

Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology Pub Date : 2002-03-25 DOI:10.1016/S0167-4838(01)00338-7

Patrick Tauc , C.Reyes Mateo , Jean-Claude Brochon

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引用次数: 16

摘要

动态荧光光谱技术为研究中高静水压力下生物分子的功能和结构变化提供了新的视角。简要介绍了时间分辨荧光方法的原理，并总结了所得信息的类型。在变性、三元和四元结构、聚集以及与DNA的相互作用方面，介绍了动态荧光对蛋白质压力效应的第一组选择应用。第二组应用致力于压力和胆固醇对脂质膜横向非均质性的影响。

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Investigation of the effect of high hydrostatic pressure on proteins and lipidic membranes by dynamic fluorescence spectroscopy

Dynamic fluorescence spectroscopy brings new insight into the functional and structural changes of biological molecules under moderate and high hydrostatic pressure. The principles of time-resolved fluorescence methods are briefly described and the resulting type of information is summarized. A first set of selected applications of the use of dynamic fluorescence on pressure effects on proteins in terms of denaturation, ternary and quaternary structure, aggregation and also interaction with DNA are presented. A second set of applications is devoted to the effect of pressure and of cholesterol on lateral heterogeneity of lipidic membranes.

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Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology

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