x染色体rna结合基序蛋白(RBMX)是着丝点形成的必要条件

Mei Hann Lee, Linyen Lin, H. Takata, Akihiro Morimoto, S. Uchiyama, S. Matsunaga, K. Fukui
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引用次数: 0

摘要

RBMX是一种rna结合基序蛋白,被鉴定为纯化的人类中期染色体的组成部分。我们用电子显微镜(EM)结合RNA干扰(RNAi)分析了RBMX的功能意义。我们发现RBMX的消耗导致有丝分裂细胞的积累。电镜研究进一步显示,RBMX的缺失导致模糊球结构的增加,这使人联想到着丝前粒。这很可能是由于在着丝点成熟的早期阶段受到破坏,从而使着丝点分化为真正的三线体形态受到干扰。目前的EM数据阐明了RBMX在着丝点形成中对随后稳定的微管相互作用的重要性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
An X-chromosome RNA-binding motif protein (RBMX) is required for proper kinetochore formation
RBMX is an RNA-binding motif protein identified as a component of purified human metaphase chromosomes. We have analyzed the functional significance of RBMX by electron microscopy (EM) in combination with RNA interference (RNAi). We revealed that depletion of RBMX results in the accumulation of mitotic cells. EM investigation further showed that RBMX depletion leads to an increase in the fuzzy ball structure that is reminiscent of the prekinetochore. It is most probably due to the result of disruption at early stages of kinetochore maturation, so that kinetochore differentiation into the authentic trilaminar morphology is disturbed. The present EM data illuminated the importance of RBMX in kinetochore formation for subsequent stable microtubules interaction.
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