水蛭科植物nrDNA-ITS标记的分子特征

IF 0.4 4区生物学 Q4 PLANT SCIENCES

GAYANA BOTANICA Pub Date : 2021-01-01 DOI:10.35513/botlit.2021.1.6

D. K. Srivastava, Pekhna Bansal, Pradeep K. Singh, Manjit Inder Singh Saggoo

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引用次数: 0

摘要

采用通用的ITS-1和ITS-2引物对药用植物异黄酮(Isodon rugosus) nrDNA-ITS序列的分子特征进行了分析。通过计算机数据挖掘和分析，确定了46个Isodon物种ITS-1、5.8S和ITS-2区域的ITS序列。只有32种的3个区域序列都是完整的，其余14种是不完整的。rugosus Isodon发现了5个基因区，分别是18S rRNA基因(部分序列，> 1.....38)、ITS-1基因(完整序列，> 39.....236)、5.8S rRNA基因(完整序列，> 237.....400)、ITS-2基因(完整序列，> 401.....607)和大亚基26S rRNA基因(部分序列，> 608.....672)。序列区记录有可变位点(VS)、单位点(ST)和种特异性简约信息位点(PI)。利用最大似然(ML)方法得到的系统发育树也收集了这些信息。

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Molecular signature of nrDNA-ITS marker in Isodon rugosus (Lamiaceae)

Molecular features of nrDNA-ITS sequences in medicinal plant Isodon rugosus were assessed with universal ITS-1 and ITS-2 primers. ITS sequences of 46 Isodon species were defined for their respective ITS-1, 5.8S and ITS-2 regions through in-silico datamining and analysis. Only in 32 species, sequence of all the three defined regions was complete, while in the rest of 14 species was partial. Isodon rugosus revealed five genic regions, namely, 18S rRNA gene (partial sequence, > 1…..38), ITS-1 (complete sequence, > 39…..236), 5.8S rRNA gene (complete sequence, > 237…..400), ITS-2 (complete sequence, > 401…..607) and large subunit 26S rRNA gene (partial sequence, > 608…..672). The sequence regions were recorded with variable sites (VS), singleton (ST) and species-specific parsimonious-informative (PI) sites. The information was also collected in a phylogenetic tree obtained using the maximum likelihood (ML) method.

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来源期刊

GAYANA BOTANICA Agricultural and Biological Sciences-Plant Science

CiteScore

0.70

自引率

0.00%

发文量

审稿时长

6-12 weeks

期刊介绍： The journal welcomes works carried out by scientists of all nationalities, and may be written in either English or Spanish. The journal receives works in systematic, taxonomy, floristic, ecology, physiology, morphology, development, conservation, cytology and phytochemical botany.