Alteration in Antioxidant Defence System and Oxidative Stress in blood of Liver Cirrhosis

Available online at: www.isroset.org Received: 10/Jan/2019, Accepted: 11/Feb/2019, Online: 28/Feb/2019 AbstractSerum liver marker enzymes ALT, AST, ALP and GGT are generally used as liver marker enzymes however they cannot provide exact idea regarding establishment of liver disease condition because many liver cirrhotic participants indicate non-significant alteration. Present study is aimed to know the level of antioxidant defense system and oxidative stress in controls and cirrhotic participants. The cross-sectional study was carried out at tertiary centre of gastroenterologist unit at Anand. Experimental participants were divided into two groups: Control (n= 52, Age26.7±1.23 years, 39 M/ 13 F) and cirrhosis (n= 44, Age53.48±2.02 years, 34 M/10 F). All participants underwent anthropometric assessment, clinical examination, biochemical parameters, antioxidant parameters and lipid peroxidation marker or oxidative stress marker. Results of our study indicates nonconclusive increase in serum enzyme level. As an average only 42-67 % of the participants indicates increase (ALT (55%), AST (67.5%), GGT (57.5%) and ALP (42.5%)). The total antioxidant activity (Gallic acid equivalent (GAE (p<0.0001)) and Trolox equivalent (TE) (p<0.0001)) and antioxidant enzymes such as Catalase (CAT (p<0.0001)), Glutathione Peroxidase (GSHPx (p<0.0001)) and Glutathione-s-transferase (GST (p<0.0001)) were significantly decreased except superoxide dismutase (SOD (p<0.05)) during cirrhosis condition. Lipid peroxidation marker or oxidative stress marker (MDA) was significantly increased (p<0.0001) in cirrhotic participants compared to controls. The alteration in the above parameters is among all (100%) the participants. Study strongly indicates that antioxidant enzymes namely GST, GSHPx and CAT can be used as surrogate markers of oxidative stress for the cirrhosis condition.