番荔枝叶提取物的细胞毒性、总酚含量及抗氧化活性研究

NS Ibrahim, M. Abdullahi
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引用次数: 3

摘要

采用乙醇法提取野茶树叶,采用卤虾致死法、总酚含量(TPC)法和DPPH自由基清除法评价其细胞毒性和抗氧化活性。粗提物在1000 μg/mL浓度下的死亡率为73.33%,对盐水对虾幼虫的lc50值为1.59 μg/mL。与阳性对照重铬酸钾(k2cr207)相比,粗提物具有显著的毒性,其LC 50值为0.57 μg/mL。用单宁酸当量mg /g样品和没食子酸当量mg /g样品对提取物进行TPC测定,结果表明,提取物中酚类化合物含量分别为266.86±2.21 mg·TAE/g和278.60±4.21 mg·GAE/g。与标准药物、抗坏血酸(IC 50 0.563 μg/ml)和丁基羟基甲苯(IC 50 0.570 μg/ml)相比,乙醇提取物的DPPH自由基清除能力显著,ic50值为2.58 μg/ml。结果表明,乙醇提取物的抗氧化性能可能与其高总酚含量有关。关键词:茉莉,抗氧化剂,细胞毒性,总酚含量
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Cytotoxicity, Total Phenolic Contents and Antioxidant Activity of the Leaves Extract of Annona Muricata
The leaves of Annona muricata were extracted using ethanol and the extracts were evaluated for cytotoxicity using Brine Shrimp Lethality Assay, total phenolic content (TPC) and antioxidant activity using DPPH radical scavenging assay. The crude extract showed 73.33 % mortality at 1000 μg/mL concentration and its LC 50 -value was 1.59 μg/mL against brine shrimp larvae. The crude extract exhibited a significant toxicity in comparison with the positive control, the potassium dichromate (K 2 Cr 2 O 7 ) with LC 50 -value 0.57 μg/mL. The TPC assay of the extracts was expressed as mg of tannic acid equivalent per gram of sample and mg of gallic acid equivalent per gram of sample, the result showed the content of phenolic compounds as 266.86 ± 2.21 mg·TAE/g and 278.60 ± 4.21 mg·GAE/g respectively. The DPPH radical scavenging activity of the ethanol extracts displayed a signigicant free radical scavenging capacity with the IC 50 value of 2.58 μg/ml compared with the standard drugs, the ascorbic acid (IC 50 0.563 μg/mL) and the butylated hydroxytoluene (IC 50 0.570 μg/mL). The result suggested that the antioxidant property exhibited by the ethanol extract could be associated with its high total phenolic contents. Keywords : Annona muricata, Antioxidants, Cytotoxicity, Total Phenolic Contents
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