东莨菪素和类胡萝卜素对转基因高β -胡萝卜素木薯采后生理变质的影响

N. L. Edoh, J. Ukpabi, J. Igoli
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引用次数: 3

摘要

木薯块茎通常在收获后72小时内发生采收后生理变质(PPD)。本研究考察了东莨菪素和类胡萝卜素在两个转基因品种EC20-7和EC20-8中与野生品种TME-7相比,在PPD发病或延迟中的作用。采用液相色谱-质谱法测定东莨菪素和类胡萝卜素的含量。新鲜品种的东莨菪碱含量(0.10 ~ 0.20 nmol/g)与储藏木薯根的含量(12.58 ~ 14.90 nmol/g)差异不显著(P < 0.05)。EC20-7品种类胡萝卜素含量分别为6.66µg/g (α-胡萝卜素)、80.45µg/g (β-胡萝卜素)和5.98µg/g(叶黄素)。EC20-8的α-胡萝卜素、β-胡萝卜素和叶黄素含量分别为6.19、69.11和3.12µg/g。品种间α-胡萝卜素含量差异不显著(P < 0.05),叶黄素含量差异不显著(P < 0.05)。结果表明,类胡萝卜素与PPD的延迟更相关,东莨菪素含量不是PPD血管条纹或变色的主要因素。因此,木薯品种的东莨菪素含量可能不能作为确定木薯块茎PPD潜力的化学标记。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Effect of Scopoletin and Carotenoids on Postharvest Physiological Deterioration (PPD) of Transgenic High Beta Carotene Cassava
Cassava tubers suffer from postharvest physiological deterioration (PPD) which normally sets in within 72 hours of harvest. This study examines the role of scopoletin and carotenoids in the onset or delay in PPD in two transgenic varieties EC20-7 and EC20-8 compared to a wild variety TME-7. Scopoletin and carotenoids were quantified by liquid chromatography-mass spectrometry. The scopoletin content (0.10 – 0.20 nmol/g) in the fresh varieties was not significantly (P>0.05) different from the amount in stored cassava roots (12.58 – 14.90 nmol/g). The carotenoid content values in EC20-7 variety were 6.66 µg/g (α-carotene), 80.45 µg/g (β-carotene) and 5.98 µg/g (lutein). As for EC20-8, α-carotene, β-carotene and lutein values were 6.19 µg/g, 69.11 µg/g and 3.12 µg/g, respectively. There was no significant (P>0.05) difference between the varieties in α-carotene content but in their lutein content. The results indicate that carotenoids are more relevant in the delay of PPD and scopoletin content is not a major factor in PPD vascular streaking or discolouration. Hence scopoletin content of cassava varieties may not be considered as a chemical marker for determining the potential of PPD in cassava tubers.
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