{"title":"从南瓜叶细胞质提取谷氨酰胺合成酶","authors":"A.V. Pushkin , V.L. Tsuprum , T.Z. Dzhokharidze , Z.G. Evstigneeva , W.L. Kretovich","doi":"10.1016/0005-2744(81)90237-0","DOIUrl":null,"url":null,"abstract":"<div><p>Glutamine synthetase (<span>l</span>-glutamate:ammonia ligase (ADP-forming), EC 6.3.1.2) from pumpkin leaf cytosol is an oligomer that consists of eight identical monomers. The enzyme has a partial specific volume of 0.720 ml/g. The pH optimum in the presence of Mg<sup>2+</sup> is 7.2. It is shown by means of electron microscopy that the enzyme consists of elongated monomers, which are arranged with point 42 symmetry at the vertices of two squares. These squares are twisted about the 4-fold axis at 40° relative to each other.</p></div>","PeriodicalId":100159,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Enzymology","volume":"662 1","pages":"Pages 160-162"},"PeriodicalIF":0.0000,"publicationDate":"1981-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0005-2744(81)90237-0","citationCount":"4","resultStr":"{\"title\":\"Glutamine synthetase from the pumpkin leaf cytosol\",\"authors\":\"A.V. Pushkin , V.L. Tsuprum , T.Z. Dzhokharidze , Z.G. Evstigneeva , W.L. Kretovich\",\"doi\":\"10.1016/0005-2744(81)90237-0\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Glutamine synthetase (<span>l</span>-glutamate:ammonia ligase (ADP-forming), EC 6.3.1.2) from pumpkin leaf cytosol is an oligomer that consists of eight identical monomers. The enzyme has a partial specific volume of 0.720 ml/g. The pH optimum in the presence of Mg<sup>2+</sup> is 7.2. It is shown by means of electron microscopy that the enzyme consists of elongated monomers, which are arranged with point 42 symmetry at the vertices of two squares. These squares are twisted about the 4-fold axis at 40° relative to each other.</p></div>\",\"PeriodicalId\":100159,\"journal\":{\"name\":\"Biochimica et Biophysica Acta (BBA) - Enzymology\",\"volume\":\"662 1\",\"pages\":\"Pages 160-162\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1981-11-13\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/0005-2744(81)90237-0\",\"citationCount\":\"4\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Biochimica et Biophysica Acta (BBA) - Enzymology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/0005274481902370\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biochimica et Biophysica Acta (BBA) - Enzymology","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/0005274481902370","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Glutamine synthetase from the pumpkin leaf cytosol
Glutamine synthetase (l-glutamate:ammonia ligase (ADP-forming), EC 6.3.1.2) from pumpkin leaf cytosol is an oligomer that consists of eight identical monomers. The enzyme has a partial specific volume of 0.720 ml/g. The pH optimum in the presence of Mg2+ is 7.2. It is shown by means of electron microscopy that the enzyme consists of elongated monomers, which are arranged with point 42 symmetry at the vertices of two squares. These squares are twisted about the 4-fold axis at 40° relative to each other.
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