Production of endo Polysaccharides from cultivated Pleurotus eryngii fruiting bodies

Concentration. Pleurotus eryngii was cultivated on four different sterilized agro wastes as wheat, rice, sugarcane and wood. Wheat substrate gave the maximum yield of P. eryngii fruiting bodies (63.4 and 11.3 gm) for 100 gram of substrate for first flush and second flush respectively. Also, cultivation of tested mushroom on wheat substrate produced high quantity of polysaccharides (0.703 mg/ml) which was colormetrically evaluated by Phenol-Sulfuric acid method. The structure of endo-polysaccharides was studied by Fourier Transform-Infrared spectroscopy (FT-IR) was indicated the presence of O-H, C-H, C-O-C, CH2 and C-O bonds that indicated the appearance of C1-C5 by 1 H & 13 C NMR. Polysaccharides analysis with HPLC indicated that it is consisting of glucose, galactose, glucournic acid, ribose, rhamnose and mannose. Polysaccharides were obtained from Pleurotus eryngii approved high antioxidant activity by Di phenyl Pichryl hydrocarbon (DPPH) radical scavenging activity which increased by increasing the concentration of polysaccharides. The obtained polysaccharides had IC50 at 0.32 mg/ml.