pH胁迫对三叶根瘤菌和黑根根瘤菌体外存活的影响

Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Agriculture Pub Date : 2013-11-26 DOI:10.15835/BUASVMCN-AGR:9811

M. Niste, R. Vidican, I. Rotar, R. Pop

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引用次数: 3

摘要

固氮共生细菌根瘤菌可以存在于不同的土壤中，适应不同的环境条件。本研究的目的是确定pH值对三叶根瘤菌和绿根根瘤菌生长的影响。采用酵母浸膏甘露醇琼脂培养基(酵母浸膏甘露醇琼脂培养基)进行根瘤菌分离，加入HCl和NaOH调节培养基pH值为5.0、6.0、8.0和9.0。根瘤菌的最佳生长pH为中性或微碱性(pH为8)，对酸度较为敏感。与三叶根瘤菌相比，三叶根瘤菌在酸性培养基中生长较好。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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The Effect of pH Stress on the Survival of Rhizobium Trifolii and Sinorhizobium Meliloti in Vitro

Nitrogen-fixing symbiotic bacteria known as rhizobia can exist in different soils and adapt to different environmental conditions. The aim of this study was to determine the impact of pH on the growth of Rhizobium trifolii and Sinorhizobium meliloti. Rhizobial species were isolated using yeast extract mannitol agar medium) in which the pH values were adjusted to 5.0, 6.0, 8.0 and 9.0 by adding HCl and NaOH. The optimum pH for rhizobia is neutral or slightly alkaline (pH 8) and they are more sensitive to acidity. Sinorhizobium meliloti developed better in an acid medium compared to Rhizobium trifolii.

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来源期刊

Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Agriculture

自引率

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审稿时长

8 weeks