柚皮苷Bi (III)配合物与β-环糊精/小牛胸腺DNA的结合:吸收和荧光特性

Sameena Yousuf, I. V. Enoch
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引用次数: 1

摘要

制备了柚皮苷-碧(III)配合物(Narb),并对其进行了紫外可见吸收和荧光分析。通过紫外可见吸收、红外、扫描电镜和x射线衍射等技术对Narb与β-环糊精(β-CD)的包合物进行了表征。Narb与β-CD包合物的化学计量比为1:1,结合常数为5.18 × 102 mol−1 dm3。在存在和不存在β-CD的情况下,研究了Narb与小牛胸腺DNA (ctDNA)的相互作用。在β-CD不存在和β-CD存在的情况下,Narb与ctDNA的结合常数分别为1.29 × 105 mol−1 dm3和6.89 × 104 mol−1 dm3。在β-CD不存在和β-CD存在的情况下,Narb与ctDNA相互作用的Stern-Volmer常数分别为1.25 × 104 mol−1 dm3和5.10 × 103 mol−1 dm3。在β-CD存在的情况下,Narb与ctDNA相互作用的结合亲和力降低。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Binding of the Bi (III) Complex of Naringin with β-Cyclodextrin/Calf Thymus DNA: Absorption and Fluorescence Characteristics
Naringin-Bi (III) complex (Narb) was prepared and analysed by UV-Visible absorption and fluorescence measurements. The inclusion complex of Narb with β-Cyclodextrin (β-CD) was characterized by the UV-Visible absorption, Infrared, scanning dlectron microscopic, and X-ray diffractometric techniques. The stoichiometry of the inclusion complex of Narb with β-CD was 1 : 1 with a binding constant of 5.18 × 102 mol−1 dm3. The interaction of Narb with Calf Thymus DNA (ctDNA) was investigated in the presence and the absence of β-CD. The binding constants for the interaction of Narb with ctDNA in the absence and the presence of β-CD were 1.29 × 105 mol−1 dm3 and 6.89 × 104 mol−1 dm3, respectively. The Stern-Volmer constants for the interaction of Narb with ctDNA in the absence and the presence of β-CD were 1.25 × 104 mol−1 dm3 and 5.10 × 103 mol−1 dm3, respectively. The lowering of the binding affinity and the were observed for the interaction of Narb with ctDNA in the presence of β-CD.
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