三叶草叶片的植物药理学研究

Mehedi Islam, Md. Al Amin Sikder, M. Rashid, Md. Khalid Hossain
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引用次数: 1

摘要

对三叶草(Cissus trifoliata, L.)叶片甲醇提取物二氯甲烷可溶组分进行反复色谱分离纯化,共得到4个化合物,分别是β-谷甾醇(1)、β-谷酮(2)、表戊醇(3)和friedelin(4)。通过高场核磁共振(NMR)分析、与先前发表的数据比较以及尽可能与真实样品进行tlc分析,确定了分离化合物的结构。其中化合物1 ~ 4为首次从该植物中分离得到。采用尾浸法和醋酸扭体反射法分别评价三叶草甲醇提取物的中枢镇痛活性和外周镇痛活性。粗提物在200和400 mg/kg体重时具有明显的中枢和外周镇痛作用。通过盐水对虾致死性生物试验对提取物的一般毒性进行了评价,其中乙酸乙酯(LC50=0.88 μg ml-1)和二氯甲烷(LC50=0.93 μg ml-1)可溶性部分表现出相当大的活性。400 mg/kg (b.w)的粗提物也表现出显著的止泻活性,而其二氯甲烷可溶性部分表现出中等的溶栓活性。达卡大学药学院。科学21(1):69- 75,2022(6月)
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Phyto-pharmacological Investigations of Leaves of Cissus trifoliata (L.)
Repeated chromatographic separation and purification of dichloromethane soluble partitionate of methanol extract of leaves of Cissus trifoliata (L.) yielded a total of four compounds, namely β-sitosterol (1), β- sitostenone (2), epifriedelanol (3) and friedelin (4). The structures of the isolated chemical compounds were established by high field nuclear magnetic resonance (NMR) analyses, comparison with previously published data and co-TLC with authentic samples, whenever possible. Compounds 1-4 appeared to be the first time they were identified in this plant species. The methanol extract of C. trifoliata was assessed for central and peripheral analgesic activities by tail immersion and acetic acid-induced writhing reflex methods, respectively. The crude extract demonstrated significant central and peripheral analgesic efficacy at 200- and 400-mg/kg b.w. in mice model. The general toxicity of the extractives was assessed through a brine shrimp lethality bioassay, where the ethyl acetate (LC50=0.88 μg ml–1) and dichloromethane (LC50=0.93 μg ml–1) soluble fractions demonstrated considerable activity. The crude extract at 400 mg/kg (b.w.) also displayed significant antidiarrheal activity, whereas its dichloromethane soluble fractions exhibited moderate thrombolytic activity. Dhaka Univ. J. Pharm. Sci. 21(1): 69-75, 2022 (June)
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