Biogenic Silver Nanoparticles /Clusters Synthesis Characterization by Scanning Electron Microscopy and 2D-3D Topographical Analysis by Mountain -andreg;- graphical tool

The microalgae were exposed to Silver nitrate (AgNO3) solution and screened for their suitability for the production of nano-silver (nano-Ag).The intracellular and extracellular enzyme are secreted by microalgae(Phormidium sp.).The microalgae extract contain various active biomolecules such as superoxide dismutase, oxidoreductases, NADH-dependent enzymes, active bio-molecules (lipid, exopolymers, peptides), some protein and pigment phycobiliproteins play a major role in the formation of nanocluster and structures in the nanometer range, confirmed by change the brown colour indicates silver nanoparticle (Ag-NPs) synthesis. Extracted biogenic silver nanoparticles and its characterization studies which is applied on the Scanning Microscopic field to analysis the 2D, 3D topographical structure. The Mountain -®-expert graphical tool play an important role in structural prediction, stereoscopic configuration analysis, stereoscopic reconstruction, lateral analysis, Contour lines, Micro valley studies, Polynomial map, Partial, analysis, extract profile study, area of hole,fractal analysis, continuous wavelets decomposition, PSD, Abbott curve, Rk parameters, morphological envelop studies, R and w calculation, distance measurement, default mini-doc and 2D roughness analysis.