决明子叶和种子提取物的植物化学筛选及抑菌活性研究

Q4 Chemistry

Bulgarian Chemical Communications Pub Date : 2019-01-01 DOI:10.34049/bcc.51.3.5049

P. T. Q. Le

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引用次数: 3

摘要

本研究的目的是测定决明子叶和种子提取物中单宁、皂苷、蒽醌和类黄酮等生物活性物质的含量。叶片和种子提取物总多酚含量分别为59.211 mg GAE/g DW和1.816 mg GAE/g DW，抗氧化能力分别为8.14 μmol Fe/g DW和2.75 μmol Fe/g DW。采用纸片扩散法结合最小抑菌浓度(MIC)测定其抑菌活性。叶提取物在MIC为400 mg/mL时对金黄色葡萄球菌和大肠杆菌有抑制作用;MIC为800 mg/mL的肠炎链球菌和枯草芽孢杆菌。此外，种子提取物在MIC为200 mg/mL时对金黄色葡萄球菌也有抑制作用;大肠杆菌、肠炎链球菌和枯草芽孢杆菌的MIC为400 mg/mL。然而，叶提取物和种子提取物对黑蚜的生长没有抑制作用。关键词:抗菌活性，抗氧化，决明子，提取物，MIC

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Phytochemical screening and antimicrobial activity of extracts of Cassia alata L. leaves and seeds

The aim of the study is to determine the presence of some bioactive compounds in Cassia alata L. leaves and seeds extracts such as tannins, saponins, anthraquinones and flavonoids. Total polyphenol contents (TPC) of leaves and seeds extracts are 59.211 mg GAE/g DW and 1.816 mg GAE/g DW, respectively, while their antioxidant capacities (AC) are 8.14 μmol Fe/g DW and 2.75 μmol Fe/g DW, respectively. The antimicrobial activity is determined by the paper disc diffusion method combined with the minimum inhibitory concentration (MIC). Leaves extract inhibits S. aureus and E. coli at MIC of 400 mg/mL; S. enteritidis and B. subtilis at MIC of 800 mg/mL. Besides, seeds extract also inhibits S. aureus at MIC of 200 mg/mL; E. coli, S. enteritidis and B. subtilis at MIC of 400 mg/mL. However, leaves and seeds extracts of C. alata do not show any inhibitions on the growth of A. niger. Keywords: Antibacterial activity, Antioxidant, Cassia alata L., Extract, MIC.

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