GLP-1R激动剂通过PI3K/AKT信号通路对缺氧再氧化心肌细胞的保护作用

IF 0.3 4区医学 Q4 GERIATRICS & GERONTOLOGY

International Journal of Gerontology Pub Date : 2020-08-01 DOI:10.6890/IJGE.202008_14(3).0004

L. Xue, Wanqu Liu, Yingwei Sun, Guang Yu, Tian-tian Wang, Xiao Ju, M. Miao

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引用次数: 2

摘要

目的:观察GLP-1R激动剂对缺氧复氧心肌细胞的保护作用，并探讨GLP-1R/PI3K/AKT信号通路的作用。方法:将H9c2心肌细胞随机分为4组:对照组、缺氧复氧模型组(H/R)、艾塞那肽组和PI3K/AKT信号通路抑制剂组。采用CCK-8法检测心肌酶，Western blot检测Caspase-3、Bcl-2、Bax蛋白的表达。结果:艾塞那肽组细胞凋亡率明显低于H/R组。H/R组LDH、CK-MB水平高于对照组，与H/R组比较，艾塞那肽组降低，PI3K/AKT通路抑制剂组较艾塞那肽组升高。H/R组Caspase-3、Bax表达高于对照组，Bcl-2表达降低。与H/R组比较，艾塞那肽组Caspase-3、Bax水平降低，Bcl-2水平升高。与艾塞那肽组比较，PI3K/AKT通路抑制剂组Caspase-3、Bax水平显著升高，Bcl-2水平降低。结论:艾塞那肽对心肌细胞缺氧再氧化损伤有保护作用。GLP-1R/PI3K/AKT信号通路可能参与了艾塞那肽抑制心肌细胞凋亡的过程。

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Protective Effects on Hypoxia Reoxygenation Cardiomyocytes by GLP-1R Agonists via PI3K/AKT Signaling Pathway

Objective: The aim of the current study was to observe the protective effect of GLP-1R agonist on hypoxia reoxygenation cardiomyocytes and to explore the role of GLP-1R/PI3K/AKT signaling pathway. Methods: H9c2 myocardial cells were randomly divided into four groups: control group, hypoxia reoxygenation model group (H/R), Exenatide group and PI3K/AKT signaling pathway inhibitor group. CCK-8 method was used to detect myocardial enzymes, and the expression of Caspase-3, Bcl-2 and Bax proteins was detected by Western blot. Results: The apoptosis rate in exenatide group was decreased than the H/R group. The levels of LDH and CK-MB in H/R group were higher than control group, which compared with the H/R group, exenatide group were decreased, and it in PI3K/AKT pathway inhibitor group were increased than exenatide group. The expression of Caspase-3 and Bax in H/R group was higher than that in control group group, and the level of Bcl-2 was decreased. Compared with H/R group, the levels of Caspase-3 and Bax in exenatide group were decreased, and the level of Bcl-2 was increased. Compared with exenatide group, the levels of Caspase-3 and Bax were significantly increased in the PI3K/AKT pathway inhibitor group, and the level of Bcl-2 was decreased. Conclusion: Exenatide has protective effects on the cardiomyocytes during hypoxia reoxygenation injury. The GLP-1R/PI3K/AKT signaling pathway may be involved in the process that exenatide inhibiting cardiomyocyte apoptosis.

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来源期刊

International Journal of Gerontology 医学-老年医学

CiteScore

0.60

自引率

0.00%

发文量

审稿时长

6-12 weeks

期刊介绍： The Journal aims to publish original research and review papers on all fields of geriatrics and gerontology, including those dealing with critical care and emergency medicine. The IJGE aims to explore and clarify the medical science and philosophy in all fields of geriatrics and gerontology, including those in the emergency and critical care medicine. The IJGE is determined not only to be a professional journal in gerontology, but also a leading source of information for the developing field of geriatric emergency and critical care medicine. It is a pioneer in Asia. Topics in the IJGE cover the advancement of diagnosis and management in urgent, serious and chronic intractable diseases in later life, preventive medicine, long-term care of disability, ethical issues in the diseased elderly and biochemistry, cell biology, endocrinology, molecular biology, pharmacology, physiology and protein chemistry involving diseases associated with age. We did not limit the territory to only critical or emergency condition inasmuch as chronic diseases are frequently brought about by inappropriate management of acute problems.