人羊水中铅结合配体的hplc - icp - ms测定

Analytical Communications Pub Date : 1999-01-01 DOI:10.1039/A900478E

G. Hall, Xiangping Zhu, Eugene G. Martin

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引用次数: 4

摘要

报道了一种分离人羊水中铅结合配体(蛋白质、氨基酸和多肽)的方法。该方法结合了高效尺寸排斥色谱法(HPSEC)分离Pb结合配体和电感耦合等离子体质谱法(ICP-MS)进行Pb检测和定量。使用HPSEC校准的蛋白质标准，在羊水中鉴定了几种铅结合配体，包括铜蓝蛋白，前白蛋白和锌肽。

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Determination of Pb-bound ligands in human amniotic fluid by HPSEC-ICP-MS

A method to fractionate the Pb-binding ligands (proteins, amino acids and peptides) in human amniotic fluid is reported. The method combines high-performance size exclusion chromatography (HPSEC) to fractionate Pb-bound ligands with inductively coupled plasma mass spectrometry (ICP-MS) for Pb detection and quantification. Using protein standards for HPSEC calibration, several Pb-binding ligands were identified in amniotic fluid that included ceruloplasmin, pre-albumin and a Zn-peptide.

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Analytical Communications

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