系统发育分析表明，新西兰分离株与欧洲分离株相似

Q3 Agricultural and Biological Sciences

New Zealand Plant Protection Pub Date : 2021-12-17 DOI:10.30843/nzpp.2021.74.11747

K. Everett, I. Pushparajah, R. Scheper

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引用次数: 0

摘要

新树菌引起苹果树溃疡病。我们通过对新西兰菌株进行测序，确定了最初为欧洲菌株设计的聚合酶链反应引物的功效。通过系统发育分析，比较了17株新西兰分离株与2株欧洲分离株核糖体间转录间隔区和β-微管蛋白基因连接区。新西兰和欧洲分离的N. ditissima在同一个分支中，表明这些基因区域在引入新西兰后几乎没有变化。与Bt-FW135和Bt-RW284引物同源性为100%。基于对来自不同地点的17株新西兰分离株的测序，这些聚合酶链反应引物可用于扩增新西兰分离株的ditissima。

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Phylogenetic analysis shows that New Zealand isolates of Neonectria ditissima are similar to European isolates

Neonectria ditissima causes a debilitating apple tree canker disease. We determined the efficacy of polymerase chain reaction primers, originally designed for European strains, by sequencing New Zealand strains. The concatenated ribosomal inter-transcribed spacer and β-tubulin gene regions of 17 New Zealand isolates were compared with those of two European strains by phylogenetic analysis. New Zealand and European isolates of N. ditissima were in the same clade, suggesting that there has been little change in these gene regions following introduction to New Zealand. There was 100% homology with Bt-FW135 and Bt-RW284 primers. Based on sequencing 17 New Zealand isolates from several locations, these polymerase chain reaction primers can be relied upon to amplify New Zealand isolates of N. ditissima.

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来源期刊

New Zealand Plant Protection Agricultural and Biological Sciences-Insect Science

CiteScore

1.10

自引率

0.00%

发文量

期刊介绍： New Zealand Plant Protection is the journal of the New Zealand Plant Protection Society. It publishes original research papers on all aspects of biology, ecology and control of weeds, vertebrate and invertebrate pests, and pathogens and beneficial micro-organisms in agriculture, horticulture, forestry and natural ecosystems of relevance to New Zealand.