从豌豆根瘤中分离的豆科固氮根瘤菌对磷酸盐的增溶潜力

International Journal of Pure & Applied Bioscience Pub Date : 2019-10-30 DOI:10.18782/2320-7051.7842

P. Kaur

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摘要

本研究从Hoshiarpur土壤中生长的豌豆(Pisum sativum (L)) var AP-3根瘤中分离出豆科根瘤菌菌株。这些分离的固氮菌株还测试了它们在Pikovskaya (PVK)琼脂、修饰Pikovskaya (MPVK)琼脂和国家植物研究所(NBRIP)琼脂上溶解磷酸三钙(TCP)的倾向。据报道，豆科苜蓿SS-8的光晕大小在NBRIP琼脂上最大(13 mm)，在PVK琼脂上最小(4 mm)。将这些菌株分别接种于PVK和NBRIP培养液中，观察它们对TCP的溶解能力。菌株rs . leguminosarum SS-8的TCP增溶率最高(NBRIP为261 μg/ml, PVK为211 μg/ml)， pH值最大(NBRIP为4.14,PVK为4.20)

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Phosphate Solubilisation Potential of Screened Nitrogen Fixing Rhizobium leguminosarum Strains Isolated from Nodules of Pea Plant

The present study was undertaken to isolate Rhizobium leguminosarum strains from the nodules of Pea (Pisum sativum (L)) var AP-3 grown in the soil of Hoshiarpur. These isolated nitrogen fixing strains were also tested for their tendency to solubilize Tricalcium Phosphate (TCP) on Pikovskaya (PVK) agar, Modified Pikovskaya (MPVK) agar and National Botanical Research Institute (NBRIP agar). The halo size of R. leguminosarum SS-8 was reported to be biggest (13 mm) on NBRIP agar and the smallest (4 mm) in PVK agar. These strains were further inoculated in PVK and NBRIP broths, respectively to see their potentials to solubilize TCP. The strain R. leguminosarum SS-8 was observed to show maximum TCP solubilisation (261 μg/ml in NBRIP and 211 μg/ml in PVK broths) with maximum decrease in pH (4.14 in NBRIP and 4.20 in PVK

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International Journal of Pure & Applied Bioscience

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