Yoonseok Kam, Sookyung Koo, Incheol Shin, Jong H. Ahn, KeWon Kang, Cheol O. Joe
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引用次数: 1
摘要
卵受精后，100和170 kDa蛋白磷酸化水平升高。受精卵中肌醇1,4,5-三磷酸和二酰基甘油含量瞬间升高。在未受精卵匀浆中处理0.2 mM CaCl2也使100和170 kDa蛋白磷酸化。数据表明，精子信号增加卵细胞浆中的IP3含量，触发Ca2+从内部储存释放到细胞基质中，激活Ca2+依赖性蛋白激酶，这是早期胚胎发育所必需的。
Analysis of protein phosphorylation in fertilized eggs of Rana dybowskii
The phosphorylation of 100 and 170 kDa proteins was increased in response to fertilization in eggs of Rana dybowskii. There was a transient increase of inositol 1,4,5-trisphosphate and diacylglycerol in fertilized eggs. Treatment of 0.2 mM CaCl2 in unfertilized egg homogenate also phosphorylated the 100 and 170 kDa proteins. Data suggest that the sperm signal increases IP3 content in egg cytoplasm, triggering Ca2+ release from internal storage into cell matrix to activate Ca2+-dependent protein kinases, necessary for the early embryonic development.
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