第二类免疫反应t淋巴细胞及其在消防员职业活动中增强炎症的作用

Q4 Medicine
N. Bychkova, A. A. Kalashnikova, N. Kalinina
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引用次数: 0

摘要

第二种免疫反应的T淋巴细胞参与炎症的发生和加重,主要是过敏性。消防员在工作期间长期暴露于不利因素而增加的炎症可导致各种疾病的发展。消防员的免疫评价是重要的任命适当的治疗和预防传染病和过敏性疾病。本文旨在分析俄罗斯EMERCOM国家消防部门员工的免疫力指标,根据年龄和专业工作量的强度进行分析。调查对象为男性79人,平均年龄31岁,工作经验1 ~ 22年,工作强度不同。外周血中采用流式细胞术(Navios, FC 500, Beckman Coulter)检测单核细胞亚群,CD3+CD294+第2型免疫反应T淋巴细胞的相对数量。测定总免疫球蛋白E(免疫球蛋白)的浓度。从鼻分泌物中测定分泌性免疫球蛋白A (Vector Best)的含量(n = 30)。使用Statistica 12.0软件包(StatSoft)对结果进行统计处理。CD3+CD294+细胞数量增加16.5%。T淋巴细胞2的数量与年龄有直接的相关性(p < 0.05)。在工作量更大的消防员组中,CD3+CD294+细胞的数量增加了5倍(p < 0.05)。在患有任何呼吸道疾病的患者中,这一人群的增加在统计上更为常见——26%的病例对11.5%的病例。T淋巴细胞2的数量与吸烟时间有明显的直接关系(p < 0.05)。T淋巴细胞2数与总IgE浓度呈正相关(p < 0.05)。23%的消防员鼻腔分泌性IgA减少,13%的被检查者,该指标超过了参考区间的上限。在高工作量的消防员中,更经常显示该指标与参考值的偏差。在CD3+CD294+细胞数量较高的组中,经典单核细胞亚群增加(p < 0.05)。因此,在不利的服务条件下,随着消防员专业工作量的增加,对感染的保护受到抑制,呼吸道损伤加重,第二类免疫反应增加。评估外周血中2型T淋巴细胞的数量将揭示对免疫炎症的T2谱的易感性,这将有助于患者治疗的个性化方法。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
T-lymphocytes of the 2nd type of the immune response and their role in enhancing inflammation during the professional activities of firefighters
T lymphocytes of the 2nd type of immune response contribute to the development and exacerbation of inflammation, mainly allergic. Increased inflammation with prolonged exposure to adverse factors during the work of firefighters can lead to the development of various diseases. Evaluation of the immunity of firefighters is important for the appointment of adequate treatment and prevention of infectious and allergic diseases. This paper aimed to analyze the indicators of immunity in employees of the state fire service of EMERCOM of Russia, depending on the age and intensity of the professional workload. The surveyed were men (n = 79), mean age 31 years, work experience from 1 to 22 years, with different workload intensity. In peripheral blood, flow cytometry (Navios, FC 500, Beckman Coulter) was used to evaluate subpopulations of monocytes, the relative number of T lymphocytes of the 2nd type of immune response CD3+CD294+. The concentration of total immunoglobulin E (Immulite) was determined. From nasal secretions, the content of secretory immunoglobulin A (Vector Best) was evaluated (n = 30). Statistical processing of the results was performed using the Statistica 12.0 package (StatSoft). An increase in the number of CD3+CD294+ cells was observed in 16.5%. A direct correlation was found between the number of T lymphocytes 2 and the age of the examined persons (р < 0.05). In the group of firefighters with a more intense workload, an increase in the number of CD3+CD294+ cells were 5 times higher (р < 0.05). Among patients who had any disease of the respiratory tract, an increase in this population was observed statistically significantly more often – in 26% of cases versus 11.5%. A strong direct correlation was found between the number of T lymphocytes 2 and the duration of smoking experience (р < 0.05). A direct correlation was established between the number of T lymphocytes 2 and the concentration of total IgE (р < 0.05). A decrease in secretory IgA in the secret from the nasal passages was observed in 23% of firefighters, in 13% of the examined, the indicator went beyond the upper limit of the reference interval. Significantly more often revealed the deviation of this indicator from the reference values in firefighters with a high workload. An increase in the subpopulation of classical monocytes was established in the group of those examined with a high number of CD3+CD294+ cells (p < 0.05). Thus, with an increase in the professional workload of firefighters in unfavorable conditions of service, inhibition of protection to infection and aggravation of damage to the respiratory tract with an increase in the 2nd type of immune response are noted. Evaluation of the number of type 2 T lymphocytes in peripheral blood will reveal a predisposition to the T2 profile of immune inflammation, which will contribute to a personalized approach to patient management.
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来源期刊
Medical Immunology (Russia)
Medical Immunology (Russia) Medicine-Immunology and Allergy
CiteScore
0.70
自引率
0.00%
发文量
88
审稿时长
12 weeks
期刊介绍: The journal mission is to promote scientific achievements in fundamental and applied immunology to various medical fields, the publication of reviews, lectures, essays by leading domestic and foreign experts in the field of fundamental and experimental immunology, clinical immunology, allergology, immunodiagnostics and immunotherapy of infectious, allergy, autoimmune diseases and cancer.
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