H. Fujiwara, K. Matsunaga, H. Kumagai, M. Ishizuka, Y. Ohizumi
{"title":"f - 7438真菌诱导细胞凋亡的一种新药物——蛇血蛋白A","authors":"H. Fujiwara, K. Matsunaga, H. Kumagai, M. Ishizuka, Y. Ohizumi","doi":"10.1211/146080800128736312","DOIUrl":null,"url":null,"abstract":"We isolated ophiobolin A from the f-7438 fungus strain. \n \n \n \nIn the L1210 cell line, ophiobolin A (0-01-1 μM) showed cytotoxicity in a concentration-dependent manner. Morphological observations revealed that ophiobolin A (1 μM) induced shrinkage in cell soma and chromatin condensation, suggesting apoptotic cell death. Moreover, in DNA gel electrophoretic experiments, a typical apoptotic DNA ladder pattern was observed after treatment with this compound. The flow cytometric experiment indicated that ophiobolin A (0.01-1 μM) caused a concentration-dependent apoptosis in close agreement with concentrations that induced cytotoxicity in L1210 cells. \n \n \n \nThe results suggested that ophiobolin A caused the death of L1210 cells through the apoptotic process. Ophiobolin A may become a powerful pharmacological tool for studying the apoptotic mechanism.","PeriodicalId":19946,"journal":{"name":"Pharmacy and Pharmacology Communications","volume":"4 1","pages":"427-431"},"PeriodicalIF":0.0000,"publicationDate":"2000-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"32","resultStr":"{\"title\":\"Ophiobolin A, a Novel Apoptosis‐inducing Agent from Fungus Strain f‐7438\",\"authors\":\"H. Fujiwara, K. Matsunaga, H. Kumagai, M. Ishizuka, Y. Ohizumi\",\"doi\":\"10.1211/146080800128736312\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"We isolated ophiobolin A from the f-7438 fungus strain. \\n \\n \\n \\nIn the L1210 cell line, ophiobolin A (0-01-1 μM) showed cytotoxicity in a concentration-dependent manner. Morphological observations revealed that ophiobolin A (1 μM) induced shrinkage in cell soma and chromatin condensation, suggesting apoptotic cell death. Moreover, in DNA gel electrophoretic experiments, a typical apoptotic DNA ladder pattern was observed after treatment with this compound. The flow cytometric experiment indicated that ophiobolin A (0.01-1 μM) caused a concentration-dependent apoptosis in close agreement with concentrations that induced cytotoxicity in L1210 cells. \\n \\n \\n \\nThe results suggested that ophiobolin A caused the death of L1210 cells through the apoptotic process. Ophiobolin A may become a powerful pharmacological tool for studying the apoptotic mechanism.\",\"PeriodicalId\":19946,\"journal\":{\"name\":\"Pharmacy and Pharmacology Communications\",\"volume\":\"4 1\",\"pages\":\"427-431\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2000-09-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"32\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Pharmacy and Pharmacology Communications\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1211/146080800128736312\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Pharmacy and Pharmacology Communications","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1211/146080800128736312","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Ophiobolin A, a Novel Apoptosis‐inducing Agent from Fungus Strain f‐7438
We isolated ophiobolin A from the f-7438 fungus strain.
In the L1210 cell line, ophiobolin A (0-01-1 μM) showed cytotoxicity in a concentration-dependent manner. Morphological observations revealed that ophiobolin A (1 μM) induced shrinkage in cell soma and chromatin condensation, suggesting apoptotic cell death. Moreover, in DNA gel electrophoretic experiments, a typical apoptotic DNA ladder pattern was observed after treatment with this compound. The flow cytometric experiment indicated that ophiobolin A (0.01-1 μM) caused a concentration-dependent apoptosis in close agreement with concentrations that induced cytotoxicity in L1210 cells.
The results suggested that ophiobolin A caused the death of L1210 cells through the apoptotic process. Ophiobolin A may become a powerful pharmacological tool for studying the apoptotic mechanism.
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