Nadine Steinmetz, Gertraud Michl, M. Maixner, C. Hoffmann
{"title":"一种快速、廉价的高通量葡萄病毒rna提取方法","authors":"Nadine Steinmetz, Gertraud Michl, M. Maixner, C. Hoffmann","doi":"10.5073/VITIS.2020.59.35-39","DOIUrl":null,"url":null,"abstract":"The extraction of RNA from grapevine tissue is a crucial step for virus diagnostics via multiplex reverse transcription-polymerase chain reaction (mRT-PCR). Conventional methods are either time-consuming or expensive when convenient extraction kits are used. Here we present an easy, but reliable extraction method that fulfills the requirements of epidemiological research (high sample throughput with maximum accuracy). A further advantage of the protocol beside the low costs is the absence of harmful chemicals like phenol or chloroform and the possibility to manage 'high-throughput' extractions and analyses.","PeriodicalId":23613,"journal":{"name":"Vitis: Journal of Grapevine Research","volume":"147 1","pages":"35-39"},"PeriodicalIF":0.0000,"publicationDate":"2020-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":"{\"title\":\"A rapid and inexpensive RNA-extraction method for high-throughput virus detection in grapevine\",\"authors\":\"Nadine Steinmetz, Gertraud Michl, M. Maixner, C. Hoffmann\",\"doi\":\"10.5073/VITIS.2020.59.35-39\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"The extraction of RNA from grapevine tissue is a crucial step for virus diagnostics via multiplex reverse transcription-polymerase chain reaction (mRT-PCR). Conventional methods are either time-consuming or expensive when convenient extraction kits are used. Here we present an easy, but reliable extraction method that fulfills the requirements of epidemiological research (high sample throughput with maximum accuracy). A further advantage of the protocol beside the low costs is the absence of harmful chemicals like phenol or chloroform and the possibility to manage 'high-throughput' extractions and analyses.\",\"PeriodicalId\":23613,\"journal\":{\"name\":\"Vitis: Journal of Grapevine Research\",\"volume\":\"147 1\",\"pages\":\"35-39\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2020-02-13\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Vitis: Journal of Grapevine Research\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.5073/VITIS.2020.59.35-39\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Vitis: Journal of Grapevine Research","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.5073/VITIS.2020.59.35-39","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
A rapid and inexpensive RNA-extraction method for high-throughput virus detection in grapevine
The extraction of RNA from grapevine tissue is a crucial step for virus diagnostics via multiplex reverse transcription-polymerase chain reaction (mRT-PCR). Conventional methods are either time-consuming or expensive when convenient extraction kits are used. Here we present an easy, but reliable extraction method that fulfills the requirements of epidemiological research (high sample throughput with maximum accuracy). A further advantage of the protocol beside the low costs is the absence of harmful chemicals like phenol or chloroform and the possibility to manage 'high-throughput' extractions and analyses.