一种快速、廉价的高通量葡萄病毒rna提取方法

Nadine Steinmetz, Gertraud Michl, M. Maixner, C. Hoffmann
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引用次数: 1

摘要

从葡萄组织中提取RNA是通过多重逆转录聚合酶链反应(mRT-PCR)进行病毒诊断的关键步骤。当使用方便的提取试剂盒时,传统方法要么耗时要么昂贵。本文提出了一种简单、可靠的提取方法,满足流行病学研究的要求(高样本通量和最高准确性)。除了低成本之外,该方案的另一个优势是不含苯酚或氯仿等有害化学物质,并且有可能进行“高通量”提取和分析。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
A rapid and inexpensive RNA-extraction method for high-throughput virus detection in grapevine
The extraction of RNA from grapevine tissue is a crucial step for virus diagnostics via multiplex reverse transcription-polymerase chain reaction (mRT-PCR). Conventional methods are either time-consuming or expensive when convenient extraction kits are used. Here we present an easy, but reliable extraction method that fulfills the requirements of epidemiological research (high sample throughput with maximum accuracy). A further advantage of the protocol beside the low costs is the absence of harmful chemicals like phenol or chloroform and the possibility to manage 'high-throughput' extractions and analyses.
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