在麻醉医师中使用碱性彗星测定氧化应激和遗传毒性

Eman S. Shaltout, D. Yahia, N. Ebrahem
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摘要

简介:长期吸入麻醉药可能会带来不能完全排除的健康风险。研究目的:这项研究的目的是观察麻醉废气是如何影响DNA损伤和氧化损伤的。研究对象与方法:麻醉科医护人员分为两组:第一组15名从事手术10年以上的医护人员,第二组15名从事手术室工作10年以上的医护人员。另外,对照组由15名从未接触过麻醉剂的员工组成。彗星试验用于检测全血样本中DNA的损伤。血清丙二醛(MDA)值评估氧化应激,超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GPX)活性评估抗氧化反应(SOD)。结果:彗星平均变量(尾DNA百分比、橄榄尾矩、尾矩)均有所增加。第1组DNA损伤最严重。多年的麻醉气体暴露与DNA损伤之间存在重要的正相关。此外,与对照组相比,I组和II组血清GPX活性显著降低,血清MDA和SOD活性水平均升高。结论:根据研究结果,多年的麻醉作用会损害DNA和脂质过氧化。因此,手术室中安全的废麻醉气体浓度限制以及可能接触到的专业人员的预防措施非常重要。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
OXIDATIVE STRESS AND GENOTOXICITY USING ALKALINE COMET ASSAY AMONG ANESTHIOLOGISTS
Introduction: Long-term inhalation anesthetics exposure may provide health risks that cannot be completely ruled out. Aim of the study: The target of this research was to see how waste anesthetic gases influenced DNA damage and oxidative damage. Subjects and Methods: Two groups of healthcare workers from the department of anesthesiology joined in the study: Group I comprised of 15 staff who had operated for at least ten years, while Group II consisted of 15 healthcare members who had worked in operating rooms for more than ten years. In addition to control group consisted of 15 employees who had never been exposed to anesthetic agents. The comet assay was used to detect the damage of DNA in whole blood samples. Serum malondialdehyde (MDA) values were used to assess oxidant stress, and superoxide dismutase (SOD) and glutathione peroxidase (GPX) activities were used to assess antioxidant response (SOD). Results: The result indicates that the mean comet variables have increased (tail DNA percentage, olive tail moment, and tail moment). In Group I, the most DNA damage was observed. There were important positive associations between years of anesthetic gas exposure and damage to DNA. In addition, in comparison to the control group, there was a significant decrease in serum GPX activity and rises in serum levels of both MDA and SOD activity in Groups I and II. Conclusions: According to the results, years of working as an anesthetic can damage DNA and lipid peroxidation. As a result, safe waste anesthetic gas concentration limits in operating rooms, as well as precautions for professionals who might be exposed, are important.
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